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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-29060 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD147 Recombinant Rabbit Monoclonal Antibody (125)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 125
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD147 in Lane A: A431 Whole Cell Lysate (30 µg). Samples were probed using a CD147 Monoclonal Antibody (Product # MA5-29060) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:42 kDa. Observed band size:54 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060) at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 54 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells. CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) staining of CD147 in monkey stomach with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060) (1:1,000, frozen section). The image showing membrane staining of gastric gland cell. Right panel: merge with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CD147 in human esophagus with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell. Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CD147 in human gastric cancer with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CD147 in human liver with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of hepatocyte.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CD147 in human placenta with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing Positive staining trophoblast.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CD147 in human rectal cancer with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell in intestinal gland.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CD147 in human stomach with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of anti-CD147 reactivity on HeLa cells using CD147 monoclonal antibody (Product # MA5-29060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD147 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 2 µL with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060) and 15 µL of 50 % Protein G agarose. Primary antibody: CD147 Recombinant Rabbit Monoclonal Antibody (125), at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa.
