Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 11-1509-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD150 Monoclonal Antibody (A12 (7D4)), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and SLAM plays a role in activation of T and B cells. Applications Reported: This A12 (7D4) antibody has been reported for use in flow cytometric analysis. Applications Tested: The A12 (7D4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg)/test per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- A12 (7D4)
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers.
A novel receptor involved in T-cell activation.
Larochelle A, Savona M, Wiggins M, Anderson S, Ichwan B, Keyvanfar K, Morrison SJ, Dunbar CE
Blood 2011 Feb 3;117(5):1550-4
Blood 2011 Feb 3;117(5):1550-4
A novel receptor involved in T-cell activation.
Cocks BG, Chang CC, Carballido JM, Yssel H, de Vries JE, Aversa G
Nature 1995 Jul 20;376(6537):260-3
Nature 1995 Jul 20;376(6537):260-3
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD19 PE-Cy7 and CD150 FITC. As expected based on known relative expression patterns, CD150 clone A12 (7D4) stains monocytes (orange), granulocytes (blue) and a subset of lymphocytes (pink).
- Conjugate
- Green dye