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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-109301 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD40 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Daudi, MG-63, SiHa, rat spleen tissue, human tonsil tissue, SKOV-3.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD40 in Daudi cell lysate. Samples were incubated with CD40 polyclonal antibody (Product # PA5-109301), at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD40 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CD40 polyclonal antibody (Product # PA5-109301), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD40 in MG-63 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CD40 polyclonal antibody (Product # PA5-109301), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD40 in paraffin-embedded rat spleen tissue. Samples were incubated with CD40 polyclonal antibody (Product # PA5-109301), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD40 in paraffin-embedded human tonsil tissue. Samples were incubated with CD40 polyclonal antibody (Product # PA5-109301), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CD40 in MG-63 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Samples were incubated with CD40 polyclonal antibody (Product # PA5-109301) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
