GTX113016

antibody from GeneTex

Targeting: TH DYT5b

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX113016

Provider

GeneTex

Proper citation

GeneTex Cat#GTX113016, RRID:AB_1952230

Product name

Tyrosine Hydroxylase antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Understanding the Mechanism of Antidepressant-Related Sexual Dysfunction: Inhibition of Tyrosine Hydroxylase in Dopaminergic Neurons after Treatment with Paroxetine but Not with Agomelatine in Male Rats.

Santana Y, Montejo AL, Martín J, LLorca G, Bueno G, Blázquez JL
Journal of clinical medicine 2019 Jan 23;8(2)

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Cervical ganglioneuroblastoma in a new born Japanese Black calf.

Park CH, Shiwa N, Kimitsuki K, Kakizaki T, Watanabe D
The Journal of veterinary medical science 2018 May 18;80(5):755-759

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Src-dependent phosphorylation of μ-opioid receptor at Tyr(336) modulates opiate withdrawal.

Zhang L, Kibaly C, Wang YJ, Xu C, Song KY, McGarrah PW, Loh HH, Liu JG, Law PY
EMBO molecular medicine 2017 Nov;9(11):1521-1536

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Pre-administration of BAX-inhibiting peptides decrease the loss of the nigral dopaminergic neurons in rats.

Ma C, Pan Y, Yang Z, Meng Z, Sun R, Wang T, Fei Y, Fan W
Life sciences 2016 Jan 1;144:113-20

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A single prolonged stress paradigm produces enduring impairments in social bonding in monogamous prairie voles.

Arai A, Hirota Y, Miyase N, Miyata S, Young LJ, Osako Y, Yuri K, Mitsui S
Behavioural brain research 2016 Dec 15;315:83-93

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Transplanted Neural Stem Cells: Playing a Neuroprotective Role by Ceruloplasmin in the Substantia Nigra of PD Model Rats?

Xiao JJ, Yin M, Wang ZJ, Wang XP
Oxidative medicine and cellular longevity 2015;2015:618631

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Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling.

Thomas MG, Saldanha M, Mistry RJ, Dexter DT, Ramsden DB, Parsons RB
Cell death & disease 2013 Jun 13;4:e669

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine Hydroxylase antibody detects TH protein by western blot analysis.A. 30 ?g NT2D1 whole cell lysate/extractB. 30 ?g PC-3 whole cell lysate/extractC. 30 ?g U87-MG whole cell lysate/extractD. 30 ?g SK-N-SH whole cell lysate/extract7.5% SDS-PAGETyrosine Hydroxylase antibody (GTX113016) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine Hydroxylase antibody detects TH protein by western blot analysis.A. 50 ?g mouse brain lysate/extract7.5% SDS-PAGETyrosine Hydroxylase antibody (GTX113016) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine Hydroxylase antibody detects TH protein by western blot analysis. A. 30 ?g PC-12 whole cell lysate/extract 7.5% SDS-PAGE Tyrosine Hydroxylase antibody (GTX113016) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein by western blot analysis. Rat tissue extracts (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:400.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine hydroxylase antibody detects tyrosine hydroxylase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Tyrosine hydroxylase antibody [GTX113016] diluted at 1:250. Blue: DAPI

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).