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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF529 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CCL17/TARC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human TARC is observed. Neutralizes the biological activity of recombinant mouse TARC, and will also neutralize the biological activity of recombinant human TARC using a 20 fold greater Ig concentration.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cross-talk between iNKT cells and CD8 T cells in the spleen requires the IL-4/CCL17 axis for the generation of short-lived effector cells.
Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.
IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli.
Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
Valente M, Dölen Y, van Dinther E, Vimeux L, Fallet M, Feuillet V, Figdor CG
Proceedings of the National Academy of Sciences of the United States of America 2019 Dec 17;116(51):25816-25827
Proceedings of the National Academy of Sciences of the United States of America 2019 Dec 17;116(51):25816-25827
Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.
Yi S, Zhai J, Niu R, Zhu G, Wang M, Liu J, Huang H, Wang Y, Jing X, Kang L, Song W, Shi Y, Tang H
Nature communications 2018 Sep 24;9(1):3879
Nature communications 2018 Sep 24;9(1):3879
IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
Araujo-Pires AC, Vieira AE, Francisconi CF, Biguetti CC, Glowacki A, Yoshizawa S, Campanelli AP, Trombone AP, Sfeir CS, Little SR, Garlet GP
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2015 Mar;30(3):412-22
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2015 Mar;30(3):412-22
Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
Loebbermann J, Durant L, Thornton H, Johansson C, Openshaw PJ
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):2987-92
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):2987-92
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
The Journal of experimental medicine 2008 Mar 17;205(3):699-710
The Journal of experimental medicine 2008 Mar 17;205(3):699-710
CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli.
Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K
European journal of immunology 2006 Aug;36(8):2116-27
European journal of immunology 2006 Aug;36(8):2116-27
Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
Carpenter KJ, Hogaboam CM
Infection and immunity 2005 Nov;73(11):7198-207
Infection and immunity 2005 Nov;73(11):7198-207
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody. Recombinant Mouse CCL17/ TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF529). The ND50 is typically 0.2-0.6 µg/mL.
