R1541P

antibody from Acris Antibodies GmbH

Targeting: MAD2L2 FANCV, MAD2B, POLZ2, REV7

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1541P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1541P, RRID:AB_1004839

Product name

anti MAD2L2

Antibody type

Polyclonal

Antigen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2.

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

1.3 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

R1541P MAD2L2 antibody was used at a 1/500 dilution to detect Human MAD2L2 by Western blot. Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to Human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1/2,000 dilution of HRP conjugated Goat-anti-Rabbit IgG was used for visualization. Exposure time was 30 sec.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

R1541P MAD2L2 antibody shows strong nuclear and cytoplasmic staining of tumor cells in cancerous Human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.