Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- NB100-478 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-478, RRID:AB_10002563
- Product name
- Mouse Monoclonal PCNA Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This reacts with the interdomain connector loop of human PCNA, other not determined.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker.
Malkas LH, Herbert BS, Abdel-Aziz W, Dobrolecki LE, Liu Y, Agarwal B, Hoelz D, Badve S, Schnaper L, Arnold RJ, Mechref Y, Novotny MV, Loehrer P, Goulet RJ, Hickey RJ
Proceedings of the National Academy of Sciences of the United States of America 2006 Dec 19;103(51):19472-7
Proceedings of the National Academy of Sciences of the United States of America 2006 Dec 19;103(51):19472-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (339) [NB100-478]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (339) [NB100-478] - A: Jurkat , B: Raji, C: 293T, D: A431, E: HeLa, F: HepG2, G: H1299, H: HCT116, I: MCF-7, J: NT2D1, K: PC-3, L: U87-MG
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (339) [NB100-478] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000