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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-35031 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USF1 in mouse spleen tissue lysate (35 µg/lane) using a USF1 polyclonal antibody (Product # PA5-35031).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of USF1 showing staining in the nucleus of SK-BR-3 cells using a USF1 polyclonal antibody (Product # PA5-35031) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 µg/mL, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USF1 in formalin-fixed, paraffin-embedded human breast carcinoma using a USF1 polyclonal antibody (Product # PA5-35031) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of USF1 in MCF-7 cells (right) compared to a negative control (left) using a USF1 polyclonal antibody (Product # PA5-35031) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
