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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00007391-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007391-M01, RRID:AB_464190
- Product name
- USF1 monoclonal antibody (M01), clone 3F6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant USF1.
- Antigen sequence
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIAS
IQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQ
LDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAV
DTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVV
TTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQ
RSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERR
RRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILS
KACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQ
QVEDLKNKNLLLRAQLRHHGLEVVIKNDSN- Isotype
- IgG
- Antibody clone number
- 3F6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references A role of DNA-PK for the metabolic gene regulation in response to insulin.
Wong RH, Chang I, Hudak CS, Hyun S, Kwan HY, Sul HS
Cell 2009 Mar 20;136(6):1056-72
Cell 2009 Mar 20;136(6):1056-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- USF1 monoclonal antibody (M01), clone 3F6 Western Blot analysis of USF1 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of USF1 expression in transfected 293T cell line by USF1 monoclonal antibody (M01), clone 3F6.Lane 1: USF1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged USF1 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
