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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-15085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-BAD (Ser112) Monoclonal Antibody (G.445.9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- G.445.9
- Vial size
- 100 µL
- Concentration
- 66 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-BAD (Ser112) using a monoclonal antibody (Product # MA5-15085).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-BAD pSer112 in extracts from COS cells, untreated or TPA-treated, using Phospho-BAD pSer112 monoclonal antibody (Product # MA5-15085) (upper) or a Bad polyclonal antibody (lower).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-BAD (Ser112) using a monoclonal antibody (Product # MA5-15085).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Phospho-BAD (Ser112) Monoclonal Antibody (G.445.9) (Product # MA5-15085) and a 18 kDa band corresponding to Bcl2-associated agonist of cell death was observed across cell lines. Whole cell extracts (40 µg lysate) of A-431 (Lane 1), A-431 (Lane 2) serum starved for 24 hrs and then treated with human EGF at 25 ng/mL for 5 minutes , A-431 (Lane 3) serum starved for 24 hrs and then treated with human EGF at 25 ng/mL for 15 minutes were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-BAD pSer112 in paraffin-embedded COS cells, untreated (left) or TPA-treated (right), using a Phospho-BAD pSer112 monoclonal antibody (Product # MA5-15085) showing induced cytoplasmic staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Phospho-BAD pSer112 in untreated (blue) or TPA/Calyculin A-treated (green) COS cells using a Phospho-BAD pSer112 monoclonal antibody (Product # MA5-15085).
