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antibody from Invitrogen Antibodies
Targeting: SUMO1
GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1
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- Antibody Data
- Antigen structure
- References [132]
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- Western blot [1]
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- Product number
- 33-2400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUMO1 Monoclonal Antibody (21C7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This monoclonal antibody can be used to specifically detect the unconjugated (~17 kDa) form of SUMO-1/GMP-1, as well as proteins covalently ligated to GMP-1 (e.g., RanGAP-1). Lysates tested: Rat liver nuclear envelopes, total lysates derived from NIH 3T3 and HeLa cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 21C7
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references SUMOylation regulates Rb hyperphosphorylation and inactivation in uveal melanoma.
CPAP enhances and maintains chronic inflammation in hepatocytes to promote hepatocarcinogenesis.
Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response.
PKCε SUMOylation Is Required for Mediating the Nociceptive Signaling of Inflammatory Pain.
Olig2 SUMOylation protects against genotoxic damage response by antagonizing p53 gene targeting.
New insights into the genetic basis of premature ovarian insufficiency: Novel causative variants and candidate genes revealed by genomic sequencing.
Perturbation of maternal PIASy abundance disrupts zygotic genome activation and embryonic development via SUMOylation pathway.
Inhibiting ubiquitination causes an accumulation of SUMOylated newly synthesized nuclear proteins at PML bodies.
Impairments in age-dependent ubiquitin proteostasis and structural integrity of selective neurons by uncoupling Ran GTPase from the Ran-binding domain 3 of Ranbp2 and identification of novel mitochondrial isoforms of ubiquitin-conjugating enzyme E2I (ubc9) and Ranbp2.
Lack of androgen receptor SUMOylation results in male infertility due to epididymal dysfunction.
Acetylation of SUMO2 at lysine 11 favors the formation of non-canonical SUMO chains.
Assessing the Role of Paralog-Specific Sumoylation of HDAC1.
SUMO regulates p21Cip1 intracellular distribution and with p21Cip1 facilitates multiprotein complex formation in the nucleolus upon DNA damage.
Myogenic differentiation triggers PML nuclear body loss and DAXX relocalization to chromocentres.
Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation.
Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans.
Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response.
Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage.
SUMOylation of Rb enhances its binding with CDK2 and phosphorylation at early G1 phase.
Unique sex chromosome systems in Ellobius: How do male XX chromosomes recombine and undergo pachytene chromatin inactivation?
SUMO Ligase Protein Inhibitor of Activated STAT1 (PIAS1) Is a Constituent Promyelocytic Leukemia Nuclear Body Protein That Contributes to the Intrinsic Antiviral Immune Response to Herpes Simplex Virus 1.
Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.
Identification of SUMO E3 ligase-specific substrates using the HuProt human proteome microarray.
SUMO1 promotes Aβ production via the modulation of autophagy.
SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling.
The ATM signaling cascade promotes recombination-dependent pachytene arrest in mouse spermatocytes.
System-wide identification of wild-type SUMO-2 conjugation sites.
Deubiquitinating activity of CYLD is impaired by SUMOylation in neuroblastoma cells.
Versatile recombinant SUMOylation system for the production of SUMO-modified protein.
Electrophilic lipid mediator 15-deoxy-Δ12,14-prostaglandin j2 modifies glucocorticoid signaling via receptor SUMOylation.
Nuclear mobility and activity of FOXA1 with androgen receptor are regulated by SUMOylation.
Ubiquitin is phosphorylated by PINK1 to activate parkin.
Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system.
SUMOylation regulates the chromatin occupancy and anti-proliferative gene programs of glucocorticoid receptor.
SUMOylation inhibits FOXM1 activity and delays mitotic transition.
Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
A pathway linking oxidative stress and the Ran GTPase system in progeria.
Regulation of the DNA damage response on male meiotic sex chromosomes.
SUMOylation negatively modulates target gene occupancy of the KDM5B, a histone lysine demethylase.
Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma.
AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice.
Prostaglandin 15d-PGJ(2) inhibits androgen receptor signaling in prostate cancer cells.
Dynamic SUMOylation is linked to the activity cycles of androgen receptor in the cell nucleus.
SUMO binding by the Epstein-Barr virus protein kinase BGLF4 is crucial for BGLF4 function.
Ubiquitin-related proteins in neuronal and glial intranuclear inclusions in intranuclear inclusion body disease.
Inactivation or non-reactivation: what accounts better for the silence of sex chromosomes during mammalian male meiosis?
Progesterone regulation of progesterone receptor membrane component 1 (PGRMC1) sumoylation and transcriptional activity in spontaneously immortalized granulosa cells.
Overexpression of SUMO perturbs the growth and development of Caenorhabditis elegans.
In vivo analysis of protein sumoylation induced by a viral protein: Detection of HCMV pp71-induced Daxx sumoylation.
Sumoylation regulates nuclear localization of repressor DREAM.
Glucocorticoid-induced activation of caspase-8 protects the glucocorticoid-induced protein Gilz from proteasomal degradation and induces its binding to SUMO-1 in murine thymocytes.
Structure of Importin13-Ubc9 complex: nuclear import and release of a key regulator of sumoylation.
Proteasome inhibitors induce nucleolar aggregation of proteasome target proteins and polyadenylated RNA by altering ubiquitin availability.
Regulation of vaccinia virus E3 protein by small ubiquitin-like modifier proteins.
The defective nuclear lamina in Hutchinson-gilford progeria syndrome disrupts the nucleocytoplasmic Ran gradient and inhibits nuclear localization of Ubc9.
Incipient intranuclear inclusion body disease in a 78-year-old woman.
A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4.
Silencing of the JNK pathway maintains progesterone receptor activity in decidualizing human endometrial stromal cells exposed to oxidative stress signals.
Development and validation of a method for profiling post-translational modification activities using protein microarrays.
The SUMO pathway functions in mouse oocyte maturation.
Ret Finger Protein: An E3 Ubiquitin Ligase Juxtaposed to the XY Body in Meiosis.
SUMOylation attenuates the function of PGC-1alpha.
Sumoylation of Prox1 controls its ability to induce VEGFR3 expression and lymphatic phenotypes in endothelial cells.
Proteomics analysis of nucleolar SUMO-1 target proteins upon proteasome inhibition.
Temporal regulation of Ig gene diversification revealed by single-cell imaging.
The SUMO-E3 ligase PIAS3 targets pyruvate kinase M2.
Small ubiquitin-like modifier 1 [corrected] mediates the resistance of prosthesis-loosening fibroblast-like synoviocytes against Fas-induced apoptosis.
SUMO conjugation contributes to immune deviation in nonobese diabetic mice by suppressing c-Maf transactivation of IL-4.
RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin.
A high incidence of meiotic silencing of unsynapsed chromatin is not associated with substantial pachytene loss in heterozygous male mice carrying multiple simple robertsonian translocations.
SUMO-modified Sp3 represses transcription by provoking local heterochromatic gene silencing.
The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle.
The adenovirus E1B-55K oncoprotein induces SUMO modification of p53.
In vivo identification of human small ubiquitin-like modifier polymerization sites by high accuracy mass spectrometry and an in vitro to in vivo strategy.
SUMO-1 transiently localizes to Cajal bodies in mammalian neurons.
Identification of a new site of sumoylation on Tel (ETV6) uncovers a PIAS-dependent mode of regulating Tel function.
Characterization of a new SUMO-1 nuclear body (SNB) enriched in pCREB, CBP, c-Jun in neuron-like UR61 cells.
Immunocell-array for molecular dissection of multiple signaling pathways in mammalian cells.
Nuclear phospholipase C gamma: punctate distribution and association with the promyelocytic leukemia protein.
E2F regulates DDB2: consequences for DNA repair in Rb-deficient cells.
SUMO-1-dependent allosteric regulation of thymine DNA glycosylase alters subnuclear localization and CBP/p300 recruitment.
An acetylation/deacetylation-SUMOylation switch through a phylogenetically conserved psiKXEP motif in the tumor suppressor HIC1 regulates transcriptional repression activity.
A mammal-specific Doublesex homolog associates with male sex chromatin and is required for male meiosis.
Phosphorylation-dependent control of Pc2 SUMO E3 ligase activity by its substrate protein HIPK2.
Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone.
SUMO-1 controls the protein stability and the biological function of phosducin.
The number of PML nuclear bodies increases in early S phase by a fission mechanism.
PDSM, a motif for phosphorylation-dependent SUMO modification.
Inhibition of DNA binding by differential sumoylation of heat shock factors.
p53-independent induction of rat hepatic Mdm2 following administration of phenobarbital and pregnenolone 16alpha-carbonitrile.
Distinct and overlapping sets of SUMO-1 and SUMO-2 target proteins revealed by quantitative proteomics.
SUMO-1 modification of the major immediate-early (IE) 1 and 2 proteins of human cytomegalovirus is regulated by different mechanisms and modulates the intracellular localization of the IE1, but not IE2, protein.
Pc2-mediated sumoylation of Smad-interacting protein 1 attenuates transcriptional repression of E-cadherin.
SUMO modification negatively modulates the transcriptional activity of CREB-binding protein via the recruitment of Daxx.
SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4.
Sumoylation induced by the Arf tumor suppressor: a p53-independent function.
The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.
Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development.
Sumoylation of the net inhibitory domain (NID) is stimulated by PIAS1 and has a negative effect on the transcriptional activity of Net.
Sumoylation of internally initiated Sp3 isoforms regulates transcriptional repression via a Trichostatin A-insensitive mechanism.
Sumoylation of p45/NF-E2: nuclear positioning and transcriptional activation of the mammalian beta-like globin gene locus.
Transcriptional repression and cell death induced by nuclear aggregates of non-polyglutamine protein.
p14ARF interacts with the SUMO-conjugating enzyme Ubc9 and promotes the sumoylation of its binding partners.
Spatial distribution and function of sterol regulatory element-binding protein 1a and 2 homo- and heterodimers by in vivo two-photon imaging and spectroscopy fluorescence resonance energy transfer.
Small ubiquitin-like modifier 1 (SUMO-1) modification of the synergy control motif of Ad4 binding protein/steroidogenic factor 1 (Ad4BP/SF-1) regulates synergistic transcription between Ad4BP/SF-1 and Sox9.
The transactivating function of peroxisome proliferator-activated receptor gamma is negatively regulated by SUMO conjugation in the amino-terminal domain.
p14 Arf promotes small ubiquitin-like modifier conjugation of Werners helicase.
A mechanism for inhibiting the SUMO pathway.
SUMO-1 modification of the Wilms' tumor suppressor WT1.
Modification of the erythroid transcription factor GATA-1 by SUMO-1.
SUMOylation of the human cytomegalovirus 72-kilodalton IE1 protein facilitates expression of the 86-kilodalton IE2 protein and promotes viral replication.
SUMO-1 marks the nuclear inclusions in familial neuronal intranuclear inclusion disease.
SUMO-1 marks the nuclear inclusions in familial neuronal intranuclear inclusion disease.
Size, position and dynamic behavior of PML nuclear bodies following cell stress as a paradigm for supramolecular trafficking and assembly.
BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta.
BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta.
Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358.
SUMO-1 modification required for transformation by adenovirus type 5 early region 1B 55-kDa oncoprotein.
Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells.
A new spectrin, beta IV, has a major truncated isoform that associates with promyelocytic leukemia protein nuclear bodies and the nuclear matrix.
Bovine papillomavirus E1 protein is sumoylated by the host cell Ubc9 protein.
Covalent modification of the transactivator protein IE2-p86 of human cytomegalovirus by conjugation to the ubiquitin-homologous proteins SUMO-1 and hSMT3b.
Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.
Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.
SUMO-1 modification of bovine papillomavirus E1 protein is required for intranuclear accumulation.
PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia.
PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia.
SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation.
SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation.
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
Meng F, Yuan Y, Ren H, Yue H, Xu B, Qian J
Cancer science 2022 Feb;113(2):622-633
Cancer science 2022 Feb;113(2):622-633
CPAP enhances and maintains chronic inflammation in hepatocytes to promote hepatocarcinogenesis.
Chen RY, Yen CJ, Lin YJ, Wang JM, Tasi TF, Huang YC, Liu YW, Tsai HW, Lee MH, Hung LY
Cell death & disease 2021 Oct 22;12(11):983
Cell death & disease 2021 Oct 22;12(11):983
Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response.
Yu F, Wei J, Cui X, Yu C, Ni W, Bungert J, Wu L, He C, Qian Z
Nucleic acids research 2021 Jun 4;49(10):5779-5797
Nucleic acids research 2021 Jun 4;49(10):5779-5797
PKCε SUMOylation Is Required for Mediating the Nociceptive Signaling of Inflammatory Pain.
Zhao X, Xia B, Cheng J, Zhu MX, Li Y
Cell reports 2020 Oct 6;33(1):108191
Cell reports 2020 Oct 6;33(1):108191
Olig2 SUMOylation protects against genotoxic damage response by antagonizing p53 gene targeting.
Liu H, Weng W, Guo R, Zhou J, Xue J, Zhong S, Cheng J, Zhu MX, Pan SJ, Li Y
Cell death and differentiation 2020 Nov;27(11):3146-3161
Cell death and differentiation 2020 Nov;27(11):3146-3161
New insights into the genetic basis of premature ovarian insufficiency: Novel causative variants and candidate genes revealed by genomic sequencing.
Jaillard S, Bell K, Akloul L, Walton K, McElreavy K, Stocker WA, Beaumont M, Harrisson C, Jääskeläinen T, Palvimo JJ, Robevska G, Launay E, Satié AP, Listyasari N, Bendavid C, Sreenivasan R, Duros S, van den Bergen J, Henry C, Domin-Bernhard M, Cornevin L, Dejucq-Rainsford N, Belaud-Rotureau MA, Odent S, Ayers KL, Ravel C, Tucker EJ, Sinclair AH
Maturitas 2020 Nov;141:9-19
Maturitas 2020 Nov;141:9-19
Perturbation of maternal PIASy abundance disrupts zygotic genome activation and embryonic development via SUMOylation pathway.
Higuchi C, Yamamoto M, Shin SW, Miyamoto K, Matsumoto K
Biology open 2019 Oct 29;8(10)
Biology open 2019 Oct 29;8(10)
Inhibiting ubiquitination causes an accumulation of SUMOylated newly synthesized nuclear proteins at PML bodies.
Sha Z, Blyszcz T, González-Prieto R, Vertegaal ACO, Goldberg AL
The Journal of biological chemistry 2019 Oct 18;294(42):15218-15234
The Journal of biological chemistry 2019 Oct 18;294(42):15218-15234
Impairments in age-dependent ubiquitin proteostasis and structural integrity of selective neurons by uncoupling Ran GTPase from the Ran-binding domain 3 of Ranbp2 and identification of novel mitochondrial isoforms of ubiquitin-conjugating enzyme E2I (ubc9) and Ranbp2.
Patil H, Yoon D, Bhowmick R, Cai Y, Cho KI, Ferreira PA
Small GTPases 2019 Mar;10(2):146-161
Small GTPases 2019 Mar;10(2):146-161
Lack of androgen receptor SUMOylation results in male infertility due to epididymal dysfunction.
Zhang FP, Malinen M, Mehmood A, Lehtiniemi T, Jääskeläinen T, Niskanen EA, Korhonen H, Laiho A, Elo LL, Ohlsson C, Kotaja N, Poutanen M, Sipilä P, Palvimo JJ
Nature communications 2019 Feb 15;10(1):777
Nature communications 2019 Feb 15;10(1):777
Acetylation of SUMO2 at lysine 11 favors the formation of non-canonical SUMO chains.
Gärtner A, Wagner K, Hölper S, Kunz K, Rodriguez MS, Müller S
EMBO reports 2018 Nov;19(11)
EMBO reports 2018 Nov;19(11)
Assessing the Role of Paralog-Specific Sumoylation of HDAC1.
Citro S, Chiocca S
Methods in molecular biology (Clifton, N.J.) 2017;1510:329-337
Methods in molecular biology (Clifton, N.J.) 2017;1510:329-337
SUMO regulates p21Cip1 intracellular distribution and with p21Cip1 facilitates multiprotein complex formation in the nucleolus upon DNA damage.
Brun S, Abella N, Berciano MT, Tapia O, Jaumot M, Freire R, Lafarga M, Agell N
PloS one 2017;12(6):e0178925
PloS one 2017;12(6):e0178925
Myogenic differentiation triggers PML nuclear body loss and DAXX relocalization to chromocentres.
Salsman J, Rapkin LM, Margam NN, Duncan R, Bazett-Jones DP, Dellaire G
Cell death & disease 2017 Mar 30;8(3):e2724
Cell death & disease 2017 Mar 30;8(3):e2724
Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation.
Bian XL, Chen HZ, Yang PB, Li YP, Zhang FN, Zhang JY, Wang WJ, Zhao WX, Zhang S, Chen QT, Zheng Y, Sun XY, Wang XM, Chien KY, Wu Q
Nature communications 2017 Feb 27;8:14420
Nature communications 2017 Feb 27;8:14420
Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans.
Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K
Neuropathology : official journal of the Japanese Society of Neuropathology 2016 Oct;36(5):441-447
Neuropathology : official journal of the Japanese Society of Neuropathology 2016 Oct;36(5):441-447
Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response.
Conn KL, Wasson P, McFarlane S, Tong L, Brown JR, Grant KG, Domingues P, Boutell C
Journal of virology 2016 May;90(9):4807-4826
Journal of virology 2016 May;90(9):4807-4826
Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage.
Maure JF, Moser SC, Jaffray EG, F Alpi A, Hay RT
Scientific reports 2016 May 17;6:26178
Scientific reports 2016 May 17;6:26178
SUMOylation of Rb enhances its binding with CDK2 and phosphorylation at early G1 phase.
Meng F, Qian J, Yue H, Li X, Xue K
Cell cycle (Georgetown, Tex.) 2016 Jul 2;15(13):1724-32
Cell cycle (Georgetown, Tex.) 2016 Jul 2;15(13):1724-32
Unique sex chromosome systems in Ellobius: How do male XX chromosomes recombine and undergo pachytene chromatin inactivation?
Matveevsky S, Bakloushinskaya I, Kolomiets O
Scientific reports 2016 Jul 18;6:29949
Scientific reports 2016 Jul 18;6:29949
SUMO Ligase Protein Inhibitor of Activated STAT1 (PIAS1) Is a Constituent Promyelocytic Leukemia Nuclear Body Protein That Contributes to the Intrinsic Antiviral Immune Response to Herpes Simplex Virus 1.
Brown JR, Conn KL, Wasson P, Charman M, Tong L, Grant K, McFarlane S, Boutell C
Journal of virology 2016 Jul 1;90(13):5939-5952
Journal of virology 2016 Jul 1;90(13):5939-5952
Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.
Wang T, Xu W, Qin M, Yang Y, Bao P, Shen F, Zhang Z, Xu J
The Journal of biological chemistry 2016 Jul 1;291(27):14373-14384
The Journal of biological chemistry 2016 Jul 1;291(27):14373-14384
Identification of SUMO E3 ligase-specific substrates using the HuProt human proteome microarray.
Cox E, Uzoma I, Guzzo C, Jeong JS, Matunis M, Blackshaw S, Zhu H
Methods in molecular biology (Clifton, N.J.) 2015;1295:455-63
Methods in molecular biology (Clifton, N.J.) 2015;1295:455-63
SUMO1 promotes Aβ production via the modulation of autophagy.
Cho SJ, Yun SM, Jo C, Lee DH, Choi KJ, Song JC, Park SI, Kim YJ, Koh YH
Autophagy 2015;11(1):100-12
Autophagy 2015;11(1):100-12
SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling.
Yan Y, Ollila S, Wong IPL, Vallenius T, Palvimo JJ, Vaahtomeri K, Mäkelä TP
Nature communications 2015 Nov 30;6:8979
Nature communications 2015 Nov 30;6:8979
The ATM signaling cascade promotes recombination-dependent pachytene arrest in mouse spermatocytes.
Pacheco S, Marcet-Ortega M, Lange J, Jasin M, Keeney S, Roig I
PLoS genetics 2015 Mar;11(3):e1005017
PLoS genetics 2015 Mar;11(3):e1005017
System-wide identification of wild-type SUMO-2 conjugation sites.
Hendriks IA, D'Souza RC, Chang JG, Mann M, Vertegaal AC
Nature communications 2015 Jun 15;6:7289
Nature communications 2015 Jun 15;6:7289
Deubiquitinating activity of CYLD is impaired by SUMOylation in neuroblastoma cells.
Kobayashi T, Masoumi KC, Massoumi R
Oncogene 2015 Apr 23;34(17):2251-60
Oncogene 2015 Apr 23;34(17):2251-60
Versatile recombinant SUMOylation system for the production of SUMO-modified protein.
Weber AR, Schuermann D, Schär P
PloS one 2014;9(7):e102157
PloS one 2014;9(7):e102157
Electrophilic lipid mediator 15-deoxy-Δ12,14-prostaglandin j2 modifies glucocorticoid signaling via receptor SUMOylation.
Paakinaho V, Kaikkonen S, Levonen AL, Palvimo JJ
Molecular and cellular biology 2014 Sep;34(17):3202-13
Molecular and cellular biology 2014 Sep;34(17):3202-13
Nuclear mobility and activity of FOXA1 with androgen receptor are regulated by SUMOylation.
Sutinen P, Rahkama V, Rytinki M, Palvimo JJ
Molecular endocrinology (Baltimore, Md.) 2014 Oct;28(10):1719-28
Molecular endocrinology (Baltimore, Md.) 2014 Oct;28(10):1719-28
Ubiquitin is phosphorylated by PINK1 to activate parkin.
Koyano F, Okatsu K, Kosako H, Tamura Y, Go E, Kimura M, Kimura Y, Tsuchiya H, Yoshihara H, Hirokawa T, Endo T, Fon EA, Trempe JF, Saeki Y, Tanaka K, Matsuda N
Nature 2014 Jun 5;510(7503):162-6
Nature 2014 Jun 5;510(7503):162-6
Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system.
Ma L, Aslanian A, Sun H, Jin M, Shi Y, Yates JR 3rd, Hunter T
Molecular & cellular proteomics : MCP 2014 Jul;13(7):1659-75
Molecular & cellular proteomics : MCP 2014 Jul;13(7):1659-75
SUMOylation regulates the chromatin occupancy and anti-proliferative gene programs of glucocorticoid receptor.
Paakinaho V, Kaikkonen S, Makkonen H, Benes V, Palvimo JJ
Nucleic acids research 2014 Feb;42(3):1575-92
Nucleic acids research 2014 Feb;42(3):1575-92
SUMOylation inhibits FOXM1 activity and delays mitotic transition.
Myatt SS, Kongsema M, Man CW, Kelly DJ, Gomes AR, Khongkow P, Karunarathna U, Zona S, Langer JK, Dunsby CW, Coombes RC, French PM, Brosens JJ, Lam EW
Oncogene 2014 Aug 21;33(34):4316-29
Oncogene 2014 Aug 21;33(34):4316-29
Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, Wakabayashi K
Neuropathology : official journal of the Japanese Society of Neuropathology 2014 Apr;34(2):140-7
Neuropathology : official journal of the Japanese Society of Neuropathology 2014 Apr;34(2):140-7
Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, Wakabayashi K
Neuropathology : official journal of the Japanese Society of Neuropathology 2014 Apr;34(2):140-7
Neuropathology : official journal of the Japanese Society of Neuropathology 2014 Apr;34(2):140-7
A pathway linking oxidative stress and the Ran GTPase system in progeria.
Datta S, Snow CJ, Paschal BM
Molecular biology of the cell 2014 Apr;25(8):1202-15
Molecular biology of the cell 2014 Apr;25(8):1202-15
Regulation of the DNA damage response on male meiotic sex chromosomes.
Lu LY, Xiong Y, Kuang H, Korakavi G, Yu X
Nature communications 2013;4:2105
Nature communications 2013;4:2105
SUMOylation negatively modulates target gene occupancy of the KDM5B, a histone lysine demethylase.
Bueno MT, Richard S
Epigenetics 2013 Nov;8(11):1162-75
Epigenetics 2013 Nov;8(11):1162-75
Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma.
Kon T, Mori F, Tanji K, Miki Y, Kimura T, Wakabayashi K
Neuropathology : official journal of the Japanese Society of Neuropathology 2013 Jun;33(3):281-7
Neuropathology : official journal of the Japanese Society of Neuropathology 2013 Jun;33(3):281-7
AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice.
Schimenti KJ, Feuer SK, Griffin LB, Graham NR, Bovet CA, Hartford S, Pendola J, Lessard C, Schimenti JC, Ward JO
Genetics 2013 Jun;194(2):447-57
Genetics 2013 Jun;194(2):447-57
Prostaglandin 15d-PGJ(2) inhibits androgen receptor signaling in prostate cancer cells.
Kaikkonen S, Paakinaho V, Sutinen P, Levonen AL, Palvimo JJ
Molecular endocrinology (Baltimore, Md.) 2013 Feb;27(2):212-23
Molecular endocrinology (Baltimore, Md.) 2013 Feb;27(2):212-23
Dynamic SUMOylation is linked to the activity cycles of androgen receptor in the cell nucleus.
Rytinki M, Kaikkonen S, Sutinen P, Paakinaho V, Rahkama V, Palvimo JJ
Molecular and cellular biology 2012 Oct;32(20):4195-205
Molecular and cellular biology 2012 Oct;32(20):4195-205
SUMO binding by the Epstein-Barr virus protein kinase BGLF4 is crucial for BGLF4 function.
Li R, Wang L, Liao G, Guzzo CM, Matunis MJ, Zhu H, Hayward SD
Journal of virology 2012 May;86(10):5412-21
Journal of virology 2012 May;86(10):5412-21
Ubiquitin-related proteins in neuronal and glial intranuclear inclusions in intranuclear inclusion body disease.
Mori F, Tanji K, Odagiri S, Hattori M, Hoshikawa Y, Kono C, Yasui K, Yokoi S, Hasegawa Y, Kamitani T, Yoshida M, Wakabayashi K
Pathology international 2012 Jun;62(6):407-11
Pathology international 2012 Jun;62(6):407-11
Inactivation or non-reactivation: what accounts better for the silence of sex chromosomes during mammalian male meiosis?
Page J, de la Fuente R, Manterola M, Parra MT, Viera A, Berríos S, Fernández-Donoso R, Rufas JS
Chromosoma 2012 Jun;121(3):307-26
Chromosoma 2012 Jun;121(3):307-26
Progesterone regulation of progesterone receptor membrane component 1 (PGRMC1) sumoylation and transcriptional activity in spontaneously immortalized granulosa cells.
Peluso JJ, Lodde V, Liu X
Endocrinology 2012 Aug;153(8):3929-39
Endocrinology 2012 Aug;153(8):3929-39
Overexpression of SUMO perturbs the growth and development of Caenorhabditis elegans.
Rytinki MM, Lakso M, Pehkonen P, Aarnio V, Reisner K, Peräkylä M, Wong G, Palvimo JJ
Cellular and molecular life sciences : CMLS 2011 Oct;68(19):3219-32
Cellular and molecular life sciences : CMLS 2011 Oct;68(19):3219-32
In vivo analysis of protein sumoylation induced by a viral protein: Detection of HCMV pp71-induced Daxx sumoylation.
Hwang J, Kalejta RF
Methods (San Diego, Calif.) 2011 Oct;55(2):160-5
Methods (San Diego, Calif.) 2011 Oct;55(2):160-5
Sumoylation regulates nuclear localization of repressor DREAM.
Palczewska M, Casafont I, Ghimire K, Rojas AM, Valencia A, Lafarga M, Mellström B, Naranjo JR
Biochimica et biophysica acta 2011 May;1813(5):1050-8
Biochimica et biophysica acta 2011 May;1813(5):1050-8
Glucocorticoid-induced activation of caspase-8 protects the glucocorticoid-induced protein Gilz from proteasomal degradation and induces its binding to SUMO-1 in murine thymocytes.
Delfino DV, Spinicelli S, Pozzesi N, Pierangeli S, Velardi E, Bruscoli S, Martelli MP, Pettirossi V, Falchi L, Kang TB, Riccardi C
Cell death and differentiation 2011 Jan;18(1):183-90
Cell death and differentiation 2011 Jan;18(1):183-90
Structure of Importin13-Ubc9 complex: nuclear import and release of a key regulator of sumoylation.
Grünwald M, Bono F
The EMBO journal 2011 Jan 19;30(2):427-38
The EMBO journal 2011 Jan 19;30(2):427-38
Proteasome inhibitors induce nucleolar aggregation of proteasome target proteins and polyadenylated RNA by altering ubiquitin availability.
Latonen L, Moore HM, Bai B, Jäämaa S, Laiho M
Oncogene 2011 Feb 17;30(7):790-805
Oncogene 2011 Feb 17;30(7):790-805
Regulation of vaccinia virus E3 protein by small ubiquitin-like modifier proteins.
González-Santamaría J, Campagna M, García MA, Marcos-Villar L, González D, Gallego P, Lopitz-Otsoa F, Guerra S, Rodríguez MS, Esteban M, Rivas C
Journal of virology 2011 Dec;85(24):12890-900
Journal of virology 2011 Dec;85(24):12890-900
The defective nuclear lamina in Hutchinson-gilford progeria syndrome disrupts the nucleocytoplasmic Ran gradient and inhibits nuclear localization of Ubc9.
Kelley JB, Datta S, Snow CJ, Chatterjee M, Ni L, Spencer A, Yang CS, Cubeñas-Potts C, Matunis MJ, Paschal BM
Molecular and cellular biology 2011 Aug;31(16):3378-95
Molecular and cellular biology 2011 Aug;31(16):3378-95
Incipient intranuclear inclusion body disease in a 78-year-old woman.
Mori F, Miki Y, Tanji K, Ogura E, Yagihashi N, Jensen PH, Wakabayashi K
Neuropathology : official journal of the Japanese Society of Neuropathology 2011 Apr;31(2):188-93
Neuropathology : official journal of the Japanese Society of Neuropathology 2011 Apr;31(2):188-93
A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4.
Du JX, McConnell BB, Yang VW
The Journal of biological chemistry 2010 Sep 3;285(36):28298-308
The Journal of biological chemistry 2010 Sep 3;285(36):28298-308
Silencing of the JNK pathway maintains progesterone receptor activity in decidualizing human endometrial stromal cells exposed to oxidative stress signals.
Leitao B, Jones MC, Fusi L, Higham J, Lee Y, Takano M, Goto T, Christian M, Lam EW, Brosens JJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1541-51
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1541-51
Development and validation of a method for profiling post-translational modification activities using protein microarrays.
Del Rincón SV, Rogers J, Widschwendter M, Sun D, Sieburg HB, Spruck C
PloS one 2010 Jun 28;5(6):e11332
PloS one 2010 Jun 28;5(6):e11332
The SUMO pathway functions in mouse oocyte maturation.
Wang ZB, Ou XH, Tong JS, Li S, Wei L, Ouyang YC, Hou Y, Schatten H, Sun QY
Cell cycle (Georgetown, Tex.) 2010 Jul 1;9(13):2640-6
Cell cycle (Georgetown, Tex.) 2010 Jul 1;9(13):2640-6
Ret Finger Protein: An E3 Ubiquitin Ligase Juxtaposed to the XY Body in Meiosis.
Gillot I, Matthews C, Puel D, Vidal F, Lopez P
International journal of cell biology 2009;2009:524858
International journal of cell biology 2009;2009:524858
SUMOylation attenuates the function of PGC-1alpha.
Rytinki MM, Palvimo JJ
The Journal of biological chemistry 2009 Sep 18;284(38):26184-93
The Journal of biological chemistry 2009 Sep 18;284(38):26184-93
Sumoylation of Prox1 controls its ability to induce VEGFR3 expression and lymphatic phenotypes in endothelial cells.
Pan MR, Chang TM, Chang HC, Su JL, Wang HW, Hung WC
Journal of cell science 2009 Sep 15;122(Pt 18):3358-64
Journal of cell science 2009 Sep 15;122(Pt 18):3358-64
Proteomics analysis of nucleolar SUMO-1 target proteins upon proteasome inhibition.
Matafora V, D'Amato A, Mori S, Blasi F, Bachi A
Molecular & cellular proteomics : MCP 2009 Oct;8(10):2243-55
Molecular & cellular proteomics : MCP 2009 Oct;8(10):2243-55
Temporal regulation of Ig gene diversification revealed by single-cell imaging.
Ordinario EC, Yabuki M, Larson RP, Maizels N
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4545-53
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4545-53
The SUMO-E3 ligase PIAS3 targets pyruvate kinase M2.
Spoden GA, Morandell D, Ehehalt D, Fiedler M, Jansen-Dürr P, Hermann M, Zwerschke W
Journal of cellular biochemistry 2009 May 15;107(2):293-302
Journal of cellular biochemistry 2009 May 15;107(2):293-302
Small ubiquitin-like modifier 1 [corrected] mediates the resistance of prosthesis-loosening fibroblast-like synoviocytes against Fas-induced apoptosis.
Meinecke I, Pap G, Mendoza H, Drange S, Ender S, Strietholt S, Gay RE, Seyfert C, Ink B, Gay S, Pap T, Peters MA
Arthritis and rheumatism 2009 Jul;60(7):2065-70
Arthritis and rheumatism 2009 Jul;60(7):2065-70
SUMO conjugation contributes to immune deviation in nonobese diabetic mice by suppressing c-Maf transactivation of IL-4.
Leavenworth JW, Ma X, Mo YY, Pauza ME
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 15;183(2):1110-9
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 15;183(2):1110-9
RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin.
Klein UR, Haindl M, Nigg EA, Muller S
Molecular biology of the cell 2009 Jan;20(1):410-8
Molecular biology of the cell 2009 Jan;20(1):410-8
A high incidence of meiotic silencing of unsynapsed chromatin is not associated with substantial pachytene loss in heterozygous male mice carrying multiple simple robertsonian translocations.
Manterola M, Page J, Vasco C, Berríos S, Parra MT, Viera A, Rufas JS, Zuccotti M, Garagna S, Fernández-Donoso R
PLoS genetics 2009 Aug;5(8):e1000625
PLoS genetics 2009 Aug;5(8):e1000625
SUMO-modified Sp3 represses transcription by provoking local heterochromatic gene silencing.
Stielow B, Sapetschnig A, Wink C, Krüger I, Suske G
EMBO reports 2008 Sep;9(9):899-906
EMBO reports 2008 Sep;9(9):899-906
The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle.
Schimmel J, Larsen KM, Matic I, van Hagen M, Cox J, Mann M, Andersen JS, Vertegaal AC
Molecular & cellular proteomics : MCP 2008 Nov;7(11):2107-22
Molecular & cellular proteomics : MCP 2008 Nov;7(11):2107-22
The adenovirus E1B-55K oncoprotein induces SUMO modification of p53.
Muller S, Dobner T
Cell cycle (Georgetown, Tex.) 2008 Mar 15;7(6):754-8
Cell cycle (Georgetown, Tex.) 2008 Mar 15;7(6):754-8
In vivo identification of human small ubiquitin-like modifier polymerization sites by high accuracy mass spectrometry and an in vitro to in vivo strategy.
Matic I, van Hagen M, Schimmel J, Macek B, Ogg SC, Tatham MH, Hay RT, Lamond AI, Mann M, Vertegaal ACO
Molecular & cellular proteomics : MCP 2008 Jan;7(1):132-44
Molecular & cellular proteomics : MCP 2008 Jan;7(1):132-44
SUMO-1 transiently localizes to Cajal bodies in mammalian neurons.
Navascues J, Bengoechea R, Tapia O, Casafont I, Berciano MT, Lafarga M
Journal of structural biology 2008 Aug;163(2):137-46
Journal of structural biology 2008 Aug;163(2):137-46
Identification of a new site of sumoylation on Tel (ETV6) uncovers a PIAS-dependent mode of regulating Tel function.
Roukens MG, Alloul-Ramdhani M, Vertegaal AC, Anvarian Z, Balog CI, Deelder AM, Hensbergen PJ, Baker DA
Molecular and cellular biology 2008 Apr;28(7):2342-57
Molecular and cellular biology 2008 Apr;28(7):2342-57
Characterization of a new SUMO-1 nuclear body (SNB) enriched in pCREB, CBP, c-Jun in neuron-like UR61 cells.
Navascués J, Bengoechea R, Tapia O, Vaqué JP, Lafarga M, Berciano MT
Chromosoma 2007 Oct;116(5):441-51
Chromosoma 2007 Oct;116(5):441-51
Immunocell-array for molecular dissection of multiple signaling pathways in mammalian cells.
Zanardi A, Giorgetti L, Botrugno OA, Minucci S, Milani P, Pelicci PG, Carbone R
Molecular & cellular proteomics : MCP 2007 May;6(5):939-47
Molecular & cellular proteomics : MCP 2007 May;6(5):939-47
Nuclear phospholipase C gamma: punctate distribution and association with the promyelocytic leukemia protein.
Ferguson BJ, Dovey CL, Lilley K, Wyllie AH, Rich T
Journal of proteome research 2007 May;6(5):2027-32
Journal of proteome research 2007 May;6(5):2027-32
E2F regulates DDB2: consequences for DNA repair in Rb-deficient cells.
Prost S, Lu P, Caldwell H, Harrison D
Oncogene 2007 May 24;26(24):3572-81
Oncogene 2007 May 24;26(24):3572-81
SUMO-1-dependent allosteric regulation of thymine DNA glycosylase alters subnuclear localization and CBP/p300 recruitment.
Mohan RD, Rao A, Gagliardi J, Tini M
Molecular and cellular biology 2007 Jan;27(1):229-43
Molecular and cellular biology 2007 Jan;27(1):229-43
An acetylation/deacetylation-SUMOylation switch through a phylogenetically conserved psiKXEP motif in the tumor suppressor HIC1 regulates transcriptional repression activity.
Stankovic-Valentin N, Deltour S, Seeler J, Pinte S, Vergoten G, Guérardel C, Dejean A, Leprince D
Molecular and cellular biology 2007 Apr;27(7):2661-75
Molecular and cellular biology 2007 Apr;27(7):2661-75
A mammal-specific Doublesex homolog associates with male sex chromatin and is required for male meiosis.
Kim S, Namekawa SH, Niswander LM, Ward JO, Lee JT, Bardwell VJ, Zarkower D
PLoS genetics 2007 Apr 20;3(4):e62
PLoS genetics 2007 Apr 20;3(4):e62
Phosphorylation-dependent control of Pc2 SUMO E3 ligase activity by its substrate protein HIPK2.
Roscic A, Möller A, Calzado MA, Renner F, Wimmer VC, Gresko E, Lüdi KS, Schmitz ML
Molecular cell 2006 Oct 6;24(1):77-89
Molecular cell 2006 Oct 6;24(1):77-89
Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone.
Jones MC, Fusi L, Higham JH, Abdel-Hafiz H, Horwitz KB, Lam EW, Brosens JJ
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 31;103(44):16272-7
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 31;103(44):16272-7
SUMO-1 controls the protein stability and the biological function of phosducin.
Klenk C, Humrich J, Quitterer U, Lohse MJ
The Journal of biological chemistry 2006 Mar 31;281(13):8357-64
The Journal of biological chemistry 2006 Mar 31;281(13):8357-64
The number of PML nuclear bodies increases in early S phase by a fission mechanism.
Dellaire G, Ching RW, Dehghani H, Ren Y, Bazett-Jones DP
Journal of cell science 2006 Mar 15;119(Pt 6):1026-33
Journal of cell science 2006 Mar 15;119(Pt 6):1026-33
PDSM, a motif for phosphorylation-dependent SUMO modification.
Hietakangas V, Anckar J, Blomster HA, Fujimoto M, Palvimo JJ, Nakai A, Sistonen L
Proceedings of the National Academy of Sciences of the United States of America 2006 Jan 3;103(1):45-50
Proceedings of the National Academy of Sciences of the United States of America 2006 Jan 3;103(1):45-50
Inhibition of DNA binding by differential sumoylation of heat shock factors.
Anckar J, Hietakangas V, Denessiouk K, Thiele DJ, Johnson MS, Sistonen L
Molecular and cellular biology 2006 Feb;26(3):955-64
Molecular and cellular biology 2006 Feb;26(3):955-64
p53-independent induction of rat hepatic Mdm2 following administration of phenobarbital and pregnenolone 16alpha-carbonitrile.
Nelson DM, Bhaskaran V, Foster WR, Lehman-McKeeman LD
Toxicological sciences : an official journal of the Society of Toxicology 2006 Dec;94(2):272-80
Toxicological sciences : an official journal of the Society of Toxicology 2006 Dec;94(2):272-80
Distinct and overlapping sets of SUMO-1 and SUMO-2 target proteins revealed by quantitative proteomics.
Vertegaal AC, Andersen JS, Ogg SC, Hay RT, Mann M, Lamond AI
Molecular & cellular proteomics : MCP 2006 Dec;5(12):2298-310
Molecular & cellular proteomics : MCP 2006 Dec;5(12):2298-310
SUMO-1 modification of the major immediate-early (IE) 1 and 2 proteins of human cytomegalovirus is regulated by different mechanisms and modulates the intracellular localization of the IE1, but not IE2, protein.
Sadanari H, Yamada R, Ohnishi K, Matsubara K, Tanaka J
Archives of virology 2005 Sep;150(9):1763-82
Archives of virology 2005 Sep;150(9):1763-82
Pc2-mediated sumoylation of Smad-interacting protein 1 attenuates transcriptional repression of E-cadherin.
Long J, Zuo D, Park M
The Journal of biological chemistry 2005 Oct 21;280(42):35477-89
The Journal of biological chemistry 2005 Oct 21;280(42):35477-89
SUMO modification negatively modulates the transcriptional activity of CREB-binding protein via the recruitment of Daxx.
Kuo HY, Chang CC, Jeng JC, Hu HM, Lin DY, Maul GG, Kwok RP, Shih HM
Proceedings of the National Academy of Sciences of the United States of America 2005 Nov 22;102(47):16973-8
Proceedings of the National Academy of Sciences of the United States of America 2005 Nov 22;102(47):16973-8
SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4.
Ihara M, Yamamoto H, Kikuchi A
Molecular and cellular biology 2005 May;25(9):3506-18
Molecular and cellular biology 2005 May;25(9):3506-18
Sumoylation induced by the Arf tumor suppressor: a p53-independent function.
Tago K, Chiocca S, Sherr CJ
Proceedings of the National Academy of Sciences of the United States of America 2005 May 24;102(21):7689-94
Proceedings of the National Academy of Sciences of the United States of America 2005 May 24;102(21):7689-94
The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.
Lee MB, Lebedeva LA, Suzawa M, Wadekar SA, Desclozeaux M, Ingraham HA
Molecular and cellular biology 2005 Mar;25(5):1879-90
Molecular and cellular biology 2005 Mar;25(5):1879-90
Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development.
Yamaguchi T, Sharma P, Athanasiou M, Kumar A, Yamada S, Kuehn MR
Molecular and cellular biology 2005 Jun;25(12):5171-82
Molecular and cellular biology 2005 Jun;25(12):5171-82
Sumoylation of the net inhibitory domain (NID) is stimulated by PIAS1 and has a negative effect on the transcriptional activity of Net.
Wasylyk C, Criqui-Filipe P, Wasylyk B
Oncogene 2005 Jan 27;24(5):820-8
Oncogene 2005 Jan 27;24(5):820-8
Sumoylation of internally initiated Sp3 isoforms regulates transcriptional repression via a Trichostatin A-insensitive mechanism.
Spengler ML, Kennett SB, Moorefield KS, Simmons SO, Brattain MG, Horowitz JM
Cellular signalling 2005 Feb;17(2):153-66
Cellular signalling 2005 Feb;17(2):153-66
Sumoylation of p45/NF-E2: nuclear positioning and transcriptional activation of the mammalian beta-like globin gene locus.
Shyu YC, Lee TL, Ting CY, Wen SC, Hsieh LJ, Li YC, Hwang JL, Lin CC, Shen CK
Molecular and cellular biology 2005 Dec;25(23):10365-78
Molecular and cellular biology 2005 Dec;25(23):10365-78
Transcriptional repression and cell death induced by nuclear aggregates of non-polyglutamine protein.
Fu L, Gao YS, Sztul E
Neurobiology of disease 2005 Dec;20(3):656-65
Neurobiology of disease 2005 Dec;20(3):656-65
p14ARF interacts with the SUMO-conjugating enzyme Ubc9 and promotes the sumoylation of its binding partners.
Rizos H, Woodruff S, Kefford RF
Cell cycle (Georgetown, Tex.) 2005 Apr;4(4):597-603
Cell cycle (Georgetown, Tex.) 2005 Apr;4(4):597-603
Spatial distribution and function of sterol regulatory element-binding protein 1a and 2 homo- and heterodimers by in vivo two-photon imaging and spectroscopy fluorescence resonance energy transfer.
Zoumi A, Datta S, Liaw LH, Wu CJ, Manthripragada G, Osborne TF, Lamorte VJ
Molecular and cellular biology 2005 Apr;25(8):2946-56
Molecular and cellular biology 2005 Apr;25(8):2946-56
Small ubiquitin-like modifier 1 (SUMO-1) modification of the synergy control motif of Ad4 binding protein/steroidogenic factor 1 (Ad4BP/SF-1) regulates synergistic transcription between Ad4BP/SF-1 and Sox9.
Komatsu T, Mizusaki H, Mukai T, Ogawa H, Baba D, Shirakawa M, Hatakeyama S, Nakayama KI, Yamamoto H, Kikuchi A, Morohashi K
Molecular endocrinology (Baltimore, Md.) 2004 Oct;18(10):2451-62
Molecular endocrinology (Baltimore, Md.) 2004 Oct;18(10):2451-62
The transactivating function of peroxisome proliferator-activated receptor gamma is negatively regulated by SUMO conjugation in the amino-terminal domain.
Yamashita D, Yamaguchi T, Shimizu M, Nakata N, Hirose F, Osumi T
Genes to cells : devoted to molecular & cellular mechanisms 2004 Nov;9(11):1017-29
Genes to cells : devoted to molecular & cellular mechanisms 2004 Nov;9(11):1017-29
p14 Arf promotes small ubiquitin-like modifier conjugation of Werners helicase.
Woods YL, Xirodimas DP, Prescott AR, Sparks A, Lane DP, Saville MK
The Journal of biological chemistry 2004 Nov 26;279(48):50157-66
The Journal of biological chemistry 2004 Nov 26;279(48):50157-66
A mechanism for inhibiting the SUMO pathway.
Boggio R, Colombo R, Hay RT, Draetta GF, Chiocca S
Molecular cell 2004 Nov 19;16(4):549-61
Molecular cell 2004 Nov 19;16(4):549-61
SUMO-1 modification of the Wilms' tumor suppressor WT1.
Smolen GA, Vassileva MT, Wells J, Matunis MJ, Haber DA
Cancer research 2004 Nov 1;64(21):7846-51
Cancer research 2004 Nov 1;64(21):7846-51
Modification of the erythroid transcription factor GATA-1 by SUMO-1.
Collavin L, Gostissa M, Avolio F, Secco P, Ronchi A, Santoro C, Del Sal G
Proceedings of the National Academy of Sciences of the United States of America 2004 Jun 15;101(24):8870-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Jun 15;101(24):8870-5
SUMOylation of the human cytomegalovirus 72-kilodalton IE1 protein facilitates expression of the 86-kilodalton IE2 protein and promotes viral replication.
Nevels M, Brune W, Shenk T
Journal of virology 2004 Jul;78(14):7803-12
Journal of virology 2004 Jul;78(14):7803-12
SUMO-1 marks the nuclear inclusions in familial neuronal intranuclear inclusion disease.
Pountney DL, Huang Y, Burns RJ, Haan E, Thompson PD, Blumbergs PC, Gai WP
Experimental neurology 2003 Nov;184(1):436-46
Experimental neurology 2003 Nov;184(1):436-46
SUMO-1 marks the nuclear inclusions in familial neuronal intranuclear inclusion disease.
Pountney DL, Huang Y, Burns RJ, Haan E, Thompson PD, Blumbergs PC, Gai WP
Experimental neurology 2003 Nov;184(1):436-46
Experimental neurology 2003 Nov;184(1):436-46
Size, position and dynamic behavior of PML nuclear bodies following cell stress as a paradigm for supramolecular trafficking and assembly.
Eskiw CH, Dellaire G, Mymryk JS, Bazett-Jones DP
Journal of cell science 2003 Nov 1;116(Pt 21):4455-66
Journal of cell science 2003 Nov 1;116(Pt 21):4455-66
BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta.
Xu L, Yang L, Moitra PK, Hashimoto K, Rallabhandi P, Kaul S, Meroni G, Jensen JP, Weissman AM, D'Arpa P
Experimental cell research 2003 Aug 1;288(1):84-93
Experimental cell research 2003 Aug 1;288(1):84-93
BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta.
Xu L, Yang L, Moitra PK, Hashimoto K, Rallabhandi P, Kaul S, Meroni G, Jensen JP, Weissman AM, D'Arpa P
Experimental cell research 2003 Aug 1;288(1):84-93
Experimental cell research 2003 Aug 1;288(1):84-93
Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358.
Saitoh H, Pizzi MD, Wang J
The Journal of biological chemistry 2002 Feb 15;277(7):4755-63
The Journal of biological chemistry 2002 Feb 15;277(7):4755-63
SUMO-1 modification required for transformation by adenovirus type 5 early region 1B 55-kDa oncoprotein.
Endter C, Kzhyshkowska J, Stauber R, Dobner T
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11312-7
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11312-7
Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells.
Kinoshita Y, Jarell AD, Flaman JM, Foltz G, Schuster J, Sopher BL, Irvin DK, Kanning K, Kornblum HI, Nelson PS, Hieter P, Morrison RS
The Journal of biological chemistry 2001 Mar 2;276(9):6656-65
The Journal of biological chemistry 2001 Mar 2;276(9):6656-65
A new spectrin, beta IV, has a major truncated isoform that associates with promyelocytic leukemia protein nuclear bodies and the nuclear matrix.
Tse WT, Tang J, Jin O, Korsgren C, John KM, Kung AL, Gwynn B, Peters LL, Lux SE
The Journal of biological chemistry 2001 Jun 29;276(26):23974-85
The Journal of biological chemistry 2001 Jun 29;276(26):23974-85
Bovine papillomavirus E1 protein is sumoylated by the host cell Ubc9 protein.
Rangasamy D, Wilson VG
The Journal of biological chemistry 2000 Sep 29;275(39):30487-95
The Journal of biological chemistry 2000 Sep 29;275(39):30487-95
Covalent modification of the transactivator protein IE2-p86 of human cytomegalovirus by conjugation to the ubiquitin-homologous proteins SUMO-1 and hSMT3b.
Hofmann H, Flöss S, Stamminger T
Journal of virology 2000 Mar;74(6):2510-24
Journal of virology 2000 Mar;74(6):2510-24
Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.
Saitoh H, Hinchey J
The Journal of biological chemistry 2000 Mar 3;275(9):6252-8
The Journal of biological chemistry 2000 Mar 3;275(9):6252-8
Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.
Saitoh H, Hinchey J
The Journal of biological chemistry 2000 Mar 3;275(9):6252-8
The Journal of biological chemistry 2000 Mar 3;275(9):6252-8
SUMO-1 modification of bovine papillomavirus E1 protein is required for intranuclear accumulation.
Rangasamy D, Woytek K, Khan SA, Wilson VG
The Journal of biological chemistry 2000 Dec 1;275(48):37999-8004
The Journal of biological chemistry 2000 Dec 1;275(48):37999-8004
PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia.
Sternsdorf T, Puccetti E, Jensen K, Hoelzer D, Will H, Ottmann OG, Ruthardt M
Molecular and cellular biology 1999 Jul;19(7):5170-8
Molecular and cellular biology 1999 Jul;19(7):5170-8
PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia.
Sternsdorf T, Puccetti E, Jensen K, Hoelzer D, Will H, Ottmann OG, Ruthardt M
Molecular and cellular biology 1999 Jul;19(7):5170-8
Molecular and cellular biology 1999 Jul;19(7):5170-8
SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation.
Desterro JM, Rodriguez MS, Hay RT
Molecular cell 1998 Aug;2(2):233-9
Molecular cell 1998 Aug;2(2):233-9
SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation.
Desterro JM, Rodriguez MS, Hay RT
Molecular cell 1998 Aug;2(2):233-9
Molecular cell 1998 Aug;2(2):233-9
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
Matunis MJ, Coutavas E, Blobel G
The Journal of cell biology 1996 Dec;135(6 Pt 1):1457-70
The Journal of cell biology 1996 Dec;135(6 Pt 1):1457-70
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
Matunis MJ, Coutavas E, Blobel G
The Journal of cell biology 1996 Dec;135(6 Pt 1):1457-70
The Journal of cell biology 1996 Dec;135(6 Pt 1):1457-70
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GMP-1 modified RanGAP-1 (90kDa) protein in rat liver nuclei using Ms x GMP-1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 In vivo validation of substrates ubiquitylated on protein microarrays. (A) Ten putative substrates of ubiquitylation identified on the protein microarrays but not reported in the literature were selected for validation of the modification in vivo . Myc- or GST-substrates were co-expressed with HA-ubiquitin in HEK293T cells. HEK293T cell extracts were prepared using denaturing conditions, substrates immunoprecipitated with anti-Myc or anti-GST antibodies, and ubiquitylation detected by immunoblotting with anti-HA antibodies. Empty vector co-expressed with HA-tagged ubiquitin served as control. Substrates indicated in each lane are: 1- ADRBK2, 2- ACVR1B, 3- PIM2, 4- PRKCgamma, 5- KIF2C, 6- RPS6KA5, 7- ITK, 8- EPHA1, 9- TRIM52, and 10- EPHA5. Of 10 substrates 8 were found to be expressed and immunoprecipitated at detectable levels and of these all demonstrated evidence of ubiquitylation in vivo . To best visualize an ubiquitin smear, substrates 1, 2, 3, 4 were separated by 10% SDS-PAGE gels, while larger molecular weight substrates 5, 6, 7, 8 were separated by 6% SDS-PAGE gels. (B) Ubiquitylation of YY1. HEK293T cells were transfected with plasmids that express HA-ubiquitin, endogenous YY1 protein immunoprecipitated from the denatured extracts, and conjugation to ubiquitin determined by Western blot analysis with anti-HA antibodies (left). Immunoprecipitation efficiency was determined by probing blots with anti-YY1 antibodies (right). Immunoprecipitation with IgG antibo
