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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- A-714 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SUMO Pan Specific Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody detects endogenous, human SUMOylated proteins in Western blots, and has similar sensitivity when tested against purified, recombinant SUMO1, SUMO2, SUMO3 and SUMO4.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P61956- Isotype
- IgG
- Antibody clone number
- 852721
- Vial size
- 100 ug
- Storage
- Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human SUMO by Western Blot. Western blot shows lysates of pan-SUMO. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO Pan Specific Monoclonal Antibody (Catalog # A-714) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
