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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG54890 - Provider product page
- Provider
- Arigo
- Product name
- anti-SUMO-1 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 55-86 (C-terminus) of Human SUMO1.
- Description
- Purification with Protein G.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 24 hrs after FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels) transfected COS-7 cells untreated (-) or treated (+) with H2O2. Top panels: cell lysates stained with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by stained with ARG54890 anti-SUMO-1 antibody by WB. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by stained with ARG54893 anti-SUMO-2 / SUMO-3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: GST-SUMO1 fusion protein stained with ARG54890 anti-SUMO-1 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human cancer tissue stained with ARG54890 anti-SUMO-1 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG54890 anti-SUMO-1 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
