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antibody from Invitrogen Antibodies
Targeting: SUMO1
GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-24560 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUMO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SUMO1 polyclonal antibody (Product # PA5-24560) in MCF-7 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A375 cells using a SUMO1 polyclonal antibody (Product # PA5-24560) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SUMO1 (Small ubiquitin-related modifier 1) was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SUMO1 Polyclonal Antibody (Product # PA5-24560) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing punctate staining in the nucleus and nucleoplasm localization for SUMO1. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification with CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7C1115LZR).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SUMO1 was achieved by transfecting Hep G2 cells with SUMO1 specific siRNA (Silencer® select Product # s14608, s14609). Immunofluorescence analysis was performed on untransfected Hep G2 cells, transfected with non-specific scrambled siRNA, and transfected with SUMO1, SUMO2 and SUMO3 siRNA, separately. Cells were fixed, permeabilized, and labelled with SUMO1 Polyclonal Antibody (Product # PA5-24560, 1:100) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (Red) staining. Reduction of specific signal was observed in SUMO1 siRNA transfected cells but not in SUMO2 or SUMO3 transfected cells confirming specificity of the antibody to SUMO1 (Green). The images were captured at 60X magnification with EVOS™ M7000 Imaging System (Product # AMF7000).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A375 cells using a SUMO1 polyclonal antibody (Product # PA5-24560) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using a SUMO1 polyclonal antibody (Product # PA5-24560), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MCF-7 cells using a SUMO1 polyclonal antibody (Product # PA5-24560) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MCF-7 cells using a SUMO1 polyclonal antibody (Product # PA5-24560) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
