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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- NBP2-59222 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PPAR gamma/NR1C3 Antibody
- Antibody type
- Polyclonal
- Description
- Whole Antiserum.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59222] - Figure shows the result of 293T cells transfected with pNTAP-PPARG (lane 2) or with an empty vector (lane 1). The position of the protein of interest is indicated on the right (expected size: 58 kDa); the marker (in kDa) is shown on the left.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PPAR gamma/NR1C3 Antibody [NBP2-59222] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PPARG. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:27,600.
