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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10513 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10513, RRID:AB_11189684
- Product name
- CBL monoclonal antibody, clone 3B12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant CBL.
- Isotype
- IgG
- Antibody clone number
- 3B12
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against Raji (1), Raw 264.7 (2), K-562 (3), SK-BR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against recombinant protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA detection with CBL monoclonal antibody, clone 3B12 (Cat # MAB10513).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and human bladder cancer (B) tissues using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (blue) and negative control (red).
- Validation comment
- Flow Cytometry
