Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- MHCD0805 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 Monoclonal Antibody (3B5), APC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. This antibody recognizes the alpha chain alone as well as the alpha/beta heterodimer.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B5
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained using APC of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Functional comparison of a ""bipod"" BsAb produced using either large-scale ( A ) or high-throughput ( B ) methods. ( A ) Graph of the % CARNAVAL cell lysis vs concentration of antibody overlaid with the relative T cell activation, based on CD25 expression. The BsAb displays an EC 50 for CARNAVAL cell killing of 32 pM. ( B ) The same experiment in ( A ) was performed using the BsAb generated in small scale using high-throughput methods, and it shows the antibody has similar activity, displaying an EC 50 for CARNAVAL cell killing of 23 pM.