Antibody data
- Antibody Data
- Antigen structure
- References [43]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [7]
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Validation data
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- Product number
- MA5-13473 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 alpha Monoclonal Antibody (C8/144B)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-13473 targets CD8 in IHC (P) and FACSs applications and shows reactivity with Human samples.
- Antibody clone number
- C8/144B
- Concentration
- 0.5 mg/mL
Submitted references An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.
Combination immunotherapy with nivolumab and ipilimumab in patients with rare gynecological malignancies: results of the CA209-538 clinical trial.
Multiplexed immunofluorescence identifies high stromal CD68(+)PD-L1(+) macrophages as a predictor of improved survival in triple negative breast cancer.
Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.
Increased infiltration of regulatory T cells in hepatocellular carcinoma of patients with hepatitis B virus pre-S2 mutant.
Combination therapy with dendritic cell vaccine and programmed death ligand 1 immune checkpoint inhibitor for hepatocellular carcinoma in an orthotopic mouse model.
Prognostic Interactions between FAP+ Fibroblasts and CD8a+ T Cells in Colon Cancer.
PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma.
Quantitative, qualitative and spatial analysis of lymphocyte infiltration in periampullary and pancreatic adenocarcinoma.
Targeted Complement Inhibition at Synapses Prevents Microglial Synaptic Engulfment and Synapse Loss in Demyelinating Disease.
UV radiation recruits CD4(+)GATA3(+) and CD8(+)GATA3(+) T cells while altering the lipid microenvironment following inflammatory resolution in human skin in vivo.
Spatially resolved analyses link genomic and immune diversity and reveal unfavorable neutrophil activation in melanoma.
Poor Response to Neoadjuvant Chemotherapy Correlates with Mast Cell Infiltration in Inflammatory Breast Cancer.
Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.
Phase II study of ruxolitinib, a selective JAK1/2 inhibitor, in patients with metastatic triple-negative breast cancer.
A Functional Spatial Analysis Platform for Discovery of Immunological Interactions Predictive of Low-Grade to High-Grade Transition of Pancreatic Intraductal Papillary Mucinous Neoplasms.
Role of regulatory T cells and CD8(+) T lymphocytes in the dissemination of circulating tumor cells in primary invasive breast cancer.
RNAi-Mediated β-Catenin Inhibition Promotes T Cell Infiltration and Antitumor Activity in Combination with Immune Checkpoint Blockade.
Genomic and immune heterogeneity are associated with differential responses to therapy in melanoma.
Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues.
Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse.
APOBEC3G Expression Correlates with T-Cell Infiltration and Improved Clinical Outcomes in High-grade Serous Ovarian Carcinoma.
STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment.
Smoking is an independent association of squamous metaplasia in Chinese nasal polyps.
Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium.
Analysis of Immune Signatures in Longitudinal Tumor Samples Yields Insight into Biomarkers of Response and Mechanisms of Resistance to Immune Checkpoint Blockade.
Histopathological features of sinonasal inverted papillomas in chinese patients.
A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism.
Analysis of FoxP3+ T-regulatory cells and CD8+ T-cells in ovarian carcinoma: location and tumor infiltration patterns are key prognostic markers.
The differential diagnosis of CD8-positive ("type D") lymphomatoid papulosis.
Leukocyte composition of human breast cancer.
Viral acute lower respiratory infections impair CD8+ T cells through PD-1.
In situ B cell-mediated immune responses and tubulointerstitial inflammation in human lupus nephritis.
Memory B-cell aggregates in skin biopsy are diagnostic for primary Sjögren's syndrome.
Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging.
Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy--a case report.
Local immune alterations in oral mucosa of the edentulous patients; possible cause of teeth loss?
Homeostasis and effector function of lymphopenia-induced "memory-like" T cells in constitutively T cell-depleted mice.
BAFF-modulated repopulation of B lymphocytes in the blood and salivary glands of rituximab-treated patients with Sjögren's syndrome.
CD8 tumor-infiltrating lymphocytes are predictive of survival in muscle-invasive urothelial carcinoma.
Intraepithelial CD8+ tumor-infiltrating lymphocytes and a high CD8+/regulatory T cell ratio are associated with favorable prognosis in ovarian cancer.
Immunohistochemical analysis of orbital connective tissue specimens of patients with active Graves ophthalmopathy.
Etanercept treatment in the endotoxin-induced uveitis of rats.
Johnson BE, Creason AL, Stommel JM, Keck JM, Parmar S, Betts CB, Blucher A, Boniface C, Bucher E, Burlingame E, Camp T, Chin K, Eng J, Estabrook J, Feiler HS, Heskett MB, Hu Z, Kolodzie A, Kong BL, Labrie M, Lee J, Leyshock P, Mitri S, Patterson J, Riesterer JL, Sivagnanam S, Somers J, Sudar D, Thibault G, Weeder BR, Zheng C, Nan X, Thompson RF, Heiser LM, Spellman PT, Thomas G, Demir E, Chang YH, Coussens LM, Guimaraes AR, Corless C, Goecks J, Bergan R, Mitri Z, Mills GB, Gray JW
Cell reports. Medicine 2022 Feb 15;3(2):100525
Cell reports. Medicine 2022 Feb 15;3(2):100525
Combination immunotherapy with nivolumab and ipilimumab in patients with rare gynecological malignancies: results of the CA209-538 clinical trial.
Klein O, Kee D, Gao B, Markman B, da Gama Duarte J, Quigley L, Jackett L, Linklater R, Strickland A, Scott C, Mileshkin L, Palmer J, Carlino M, Behren A, Cebon J
Journal for immunotherapy of cancer 2021 Nov;9(11)
Journal for immunotherapy of cancer 2021 Nov;9(11)
Multiplexed immunofluorescence identifies high stromal CD68(+)PD-L1(+) macrophages as a predictor of improved survival in triple negative breast cancer.
Wang J, Browne L, Slapetova I, Shang F, Lee K, Lynch J, Beretov J, Whan R, Graham PH, Millar EKA
Scientific reports 2021 Nov 3;11(1):21608
Scientific reports 2021 Nov 3;11(1):21608
Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.
Khosravi-Maharlooei M, Li H, Hoelzl M, Zhao G, Ruiz A, Misra A, Li Y, Teteloshvili N, Nauman G, Danzl N, Ding X, Pinker EY, Obradovic A, Yang YG, Iuga A, Creusot RJ, Winchester R, Sykes M
Journal of autoimmunity 2021 May;119:102612
Journal of autoimmunity 2021 May;119:102612
Increased infiltration of regulatory T cells in hepatocellular carcinoma of patients with hepatitis B virus pre-S2 mutant.
Teng CF, Li TC, Wang T, Liao DC, Wen YH, Wu TH, Wang J, Wu HC, Shyu WC, Su IJ, Jeng LB
Scientific reports 2021 Jan 13;11(1):1136
Scientific reports 2021 Jan 13;11(1):1136
Combination therapy with dendritic cell vaccine and programmed death ligand 1 immune checkpoint inhibitor for hepatocellular carcinoma in an orthotopic mouse model.
Teng CF, Wang T, Wu TH, Lin JH, Shih FY, Shyu WC, Jeng LB
Therapeutic advances in medical oncology 2020;12:1758835920922034
Therapeutic advances in medical oncology 2020;12:1758835920922034
Prognostic Interactions between FAP+ Fibroblasts and CD8a+ T Cells in Colon Cancer.
Herrera M, Mezheyeuski A, Villabona L, Corvigno S, Strell C, Klein C, Hölzlwimmer G, Glimelius B, Masucci G, Sjöblom T, Östman A
Cancers 2020 Nov 3;12(11)
Cancers 2020 Nov 3;12(11)
PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma.
Liu XD, Kong W, Peterson CB, McGrail DJ, Hoang A, Zhang X, Lam T, Pilie PG, Zhu H, Beckermann KE, Haake SM, Isgandrova S, Martinez-Moczygemba M, Sahni N, Tannir NM, Lin SY, Rathmell WK, Jonasch E
Nature communications 2020 May 1;11(1):2135
Nature communications 2020 May 1;11(1):2135
Quantitative, qualitative and spatial analysis of lymphocyte infiltration in periampullary and pancreatic adenocarcinoma.
Lundgren S, Elebro J, Heby M, Nodin B, Leandersson K, Micke P, Jirström K, Mezheyeuski A
International journal of cancer 2020 Jun 15;146(12):3461-3473
International journal of cancer 2020 Jun 15;146(12):3461-3473
Targeted Complement Inhibition at Synapses Prevents Microglial Synaptic Engulfment and Synapse Loss in Demyelinating Disease.
Werneburg S, Jung J, Kunjamma RB, Ha SK, Luciano NJ, Willis CM, Gao G, Biscola NP, Havton LA, Crocker SJ, Popko B, Reich DS, Schafer DP
Immunity 2020 Jan 14;52(1):167-182.e7
Immunity 2020 Jan 14;52(1):167-182.e7
UV radiation recruits CD4(+)GATA3(+) and CD8(+)GATA3(+) T cells while altering the lipid microenvironment following inflammatory resolution in human skin in vivo.
Hawkshaw NJ, Pilkington SM, Murphy SA, Al-Gazaq N, Farrar MD, Watson RE, Nicolaou A, Rhodes LE
Clinical & translational immunology 2020 Apr;9(4):e01104
Clinical & translational immunology 2020 Apr;9(4):e01104
Spatially resolved analyses link genomic and immune diversity and reveal unfavorable neutrophil activation in melanoma.
Mitra A, Andrews MC, Roh W, De Macedo MP, Hudgens CW, Carapeto F, Singh S, Reuben A, Wang F, Mao X, Song X, Wani K, Tippen S, Ng KS, Schalck A, Sakellariou-Thompson DA, Chen E, Reddy SM, Spencer CN, Wiesnoski D, Little LD, Gumbs C, Cooper ZA, Burton EM, Hwu P, Davies MA, Zhang J, Bernatchez C, Navin N, Sharma P, Allison JP, Wargo JA, Yee C, Tetzlaff MT, Hwu WJ, Lazar AJ, Futreal PA
Nature communications 2020 Apr 15;11(1):1839
Nature communications 2020 Apr 15;11(1):1839
Poor Response to Neoadjuvant Chemotherapy Correlates with Mast Cell Infiltration in Inflammatory Breast Cancer.
Reddy SM, Reuben A, Barua S, Jiang H, Zhang S, Wang L, Gopalakrishnan V, Hudgens CW, Tetzlaff MT, Reuben JM, Tsujikawa T, Coussens LM, Wani K, He Y, Villareal L, Wood A, Rao A, Woodward WA, Ueno NT, Krishnamurthy S, Wargo JA, Mittendorf EA
Cancer immunology research 2019 Jun;7(6):1025-1035
Cancer immunology research 2019 Jun;7(6):1025-1035
Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.
Cassetta L, Fragkogianni S, Sims AH, Swierczak A, Forrester LM, Zhang H, Soong DYH, Cotechini T, Anur P, Lin EY, Fidanza A, Lopez-Yrigoyen M, Millar MR, Urman A, Ai Z, Spellman PT, Hwang ES, Dixon JM, Wiechmann L, Coussens LM, Smith HO, Pollard JW
Cancer cell 2019 Apr 15;35(4):588-602.e10
Cancer cell 2019 Apr 15;35(4):588-602.e10
Phase II study of ruxolitinib, a selective JAK1/2 inhibitor, in patients with metastatic triple-negative breast cancer.
Stover DG, Gil Del Alcazar CR, Brock J, Guo H, Overmoyer B, Balko J, Xu Q, Bardia A, Tolaney SM, Gelman R, Lloyd M, Wang Y, Xu Y, Michor F, Wang V, Winer EP, Polyak K, Lin NU
NPJ breast cancer 2018;4:10
NPJ breast cancer 2018;4:10
A Functional Spatial Analysis Platform for Discovery of Immunological Interactions Predictive of Low-Grade to High-Grade Transition of Pancreatic Intraductal Papillary Mucinous Neoplasms.
Barua S, Solis L, Parra ER, Uraoka N, Jiang M, Wang H, Rodriguez-Canales J, Wistuba I, Maitra A, Sen S, Rao A
Cancer informatics 2018;17:1176935118782880
Cancer informatics 2018;17:1176935118782880
Role of regulatory T cells and CD8(+) T lymphocytes in the dissemination of circulating tumor cells in primary invasive breast cancer.
Xue D, Xia T, Wang J, Chong M, Wang S, Zhang C
Oncology letters 2018 Sep;16(3):3045-3053
Oncology letters 2018 Sep;16(3):3045-3053
RNAi-Mediated β-Catenin Inhibition Promotes T Cell Infiltration and Antitumor Activity in Combination with Immune Checkpoint Blockade.
Ganesh S, Shui X, Craig KP, Park J, Wang W, Brown BD, Abrams MT
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Nov 7;26(11):2567-2579
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Nov 7;26(11):2567-2579
Genomic and immune heterogeneity are associated with differential responses to therapy in melanoma.
Reuben A, Spencer CN, Prieto PA, Gopalakrishnan V, Reddy SM, Miller JP, Mao X, De Macedo MP, Chen J, Song X, Jiang H, Chen PL, Beird HC, Garber HR, Roh W, Wani K, Chen E, Haymaker C, Forget MA, Little LD, Gumbs C, Thornton RL, Hudgens CW, Chen WS, Austin-Breneman J, Sloane RS, Nezi L, Cogdill AP, Bernatchez C, Roszik J, Hwu P, Woodman SE, Chin L, Tawbi H, Davies MA, Gershenwald JE, Amaria RN, Glitza IC, Diab A, Patel SP, Hu J, Lee JE, Grimm EA, Tetzlaff MT, Lazar AJ, Wistuba II, Clise-Dwyer K, Carter BW, Zhang J, Futreal PA, Sharma P, Allison JP, Cooper ZA, Wargo JA
NPJ genomic medicine 2017;2
NPJ genomic medicine 2017;2
Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues.
Parra ER, Uraoka N, Jiang M, Cook P, Gibbons D, Forget MA, Bernatchez C, Haymaker C, Wistuba II, Rodriguez-Canales J
Scientific reports 2017 Oct 17;7(1):13380
Scientific reports 2017 Oct 17;7(1):13380
Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse.
Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, Sugino T, Mitsuya K, Hayashi N, Nakasu Y, Maruyama K, Yamaguchi K, Katano I, Ito M, Akiyama Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jan 1;23(1):149-158
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jan 1;23(1):149-158
APOBEC3G Expression Correlates with T-Cell Infiltration and Improved Clinical Outcomes in High-grade Serous Ovarian Carcinoma.
Leonard B, Starrett GJ, Maurer MJ, Oberg AL, Van Bockstal M, Van Dorpe J, De Wever O, Helleman J, Sieuwerts AM, Berns EM, Martens JW, Anderson BD, Brown WL, Kalli KR, Kaufmann SH, Harris RS
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Sep 15;22(18):4746-55
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Sep 15;22(18):4746-55
STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment.
Koyama S, Akbay EA, Li YY, Aref AR, Skoulidis F, Herter-Sprie GS, Buczkowski KA, Liu Y, Awad MM, Denning WL, Diao L, Wang J, Parra-Cuentas ER, Wistuba II, Soucheray M, Thai T, Asahina H, Kitajima S, Altabef A, Cavanaugh JD, Rhee K, Gao P, Zhang H, Fecci PE, Shimamura T, Hellmann MD, Heymach JV, Hodi FS, Freeman GJ, Barbie DA, Dranoff G, Hammerman PS, Wong KK
Cancer research 2016 Mar 1;76(5):999-1008
Cancer research 2016 Mar 1;76(5):999-1008
Smoking is an independent association of squamous metaplasia in Chinese nasal polyps.
Gao T, Ng CL, Li C, Li YY, Duan C, Shen L, Jiao YF, Liu M, Wang de Y
International forum of allergy & rhinology 2016 Jan;6(1):66-74
International forum of allergy & rhinology 2016 Jan;6(1):66-74
Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium.
Shanmugasundaram U, Hilton JF, Critchfield JW, Greenblatt RM, Giudice LC, Averbach S, Seidman D, Shacklett BL, Smith-McCune K
American journal of reproductive immunology (New York, N.Y. : 1989) 2016 Aug;76(2):137-48
American journal of reproductive immunology (New York, N.Y. : 1989) 2016 Aug;76(2):137-48
Analysis of Immune Signatures in Longitudinal Tumor Samples Yields Insight into Biomarkers of Response and Mechanisms of Resistance to Immune Checkpoint Blockade.
Chen PL, Roh W, Reuben A, Cooper ZA, Spencer CN, Prieto PA, Miller JP, Bassett RL, Gopalakrishnan V, Wani K, De Macedo MP, Austin-Breneman JL, Jiang H, Chang Q, Reddy SM, Chen WS, Tetzlaff MT, Broaddus RJ, Davies MA, Gershenwald JE, Haydu L, Lazar AJ, Patel SP, Hwu P, Hwu WJ, Diab A, Glitza IC, Woodman SE, Vence LM, Wistuba II, Amaria RN, Kwong LN, Prieto V, Davis RE, Ma W, Overwijk WW, Sharpe AH, Hu J, Futreal PA, Blando J, Sharma P, Allison JP, Chin L, Wargo JA
Cancer discovery 2016 Aug;6(8):827-37
Cancer discovery 2016 Aug;6(8):827-37
Histopathological features of sinonasal inverted papillomas in chinese patients.
Zhao L, Li CW, Jin P, Ng CL, Lin ZB, Li YY, Li TY, Petersson BF, Shi L, Wang de Y
The Laryngoscope 2016 Apr;126(4):E141-7
The Laryngoscope 2016 Apr;126(4):E141-7
A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism.
Li G, Nguyen CC, Ryckman BJ, Britt WJ, Kamil JP
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 7;112(14):4471-6
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 7;112(14):4471-6
Analysis of FoxP3+ T-regulatory cells and CD8+ T-cells in ovarian carcinoma: location and tumor infiltration patterns are key prognostic markers.
Hermans C, Anz D, Engel J, Kirchner T, Endres S, Mayr D
PloS one 2014;9(11):e111757
PloS one 2014;9(11):e111757
The differential diagnosis of CD8-positive ("type D") lymphomatoid papulosis.
McQuitty E, Curry JL, Tetzlaff MT, Prieto VG, Duvic M, Torres-Cabala C
Journal of cutaneous pathology 2014 Feb;41(2):88-100
Journal of cutaneous pathology 2014 Feb;41(2):88-100
Leukocyte composition of human breast cancer.
Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, Coussens LM
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):2796-801
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):2796-801
Viral acute lower respiratory infections impair CD8+ T cells through PD-1.
Erickson JJ, Gilchuk P, Hastings AK, Tollefson SJ, Johnson M, Downing MB, Boyd KL, Johnson JE, Kim AS, Joyce S, Williams JV
The Journal of clinical investigation 2012 Aug;122(8):2967-82
The Journal of clinical investigation 2012 Aug;122(8):2967-82
In situ B cell-mediated immune responses and tubulointerstitial inflammation in human lupus nephritis.
Chang A, Henderson SG, Brandt D, Liu N, Guttikonda R, Hsieh C, Kaverina N, Utset TO, Meehan SM, Quigg RJ, Meffre E, Clark MR
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 1;186(3):1849-60
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 1;186(3):1849-60
Memory B-cell aggregates in skin biopsy are diagnostic for primary Sjögren's syndrome.
Roguedas AM, Pers JO, Lemasson G, Devauchelle V, Tobón GJ, Saraux A, Misery L, Youinou P
Journal of autoimmunity 2010 Nov;35(3):241-7
Journal of autoimmunity 2010 Nov;35(3):241-7
Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging.
Chen CH, Yeh YC, Wu GJ, Huang YH, Lai WF, Liu JY, Tzeng CR
Reproduction (Cambridge, England) 2010 Jul;140(1):105-12
Reproduction (Cambridge, England) 2010 Jul;140(1):105-12
Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy--a case report.
Milani V, Stangl S, Issels R, Gehrmann M, Wagner B, Hube K, Mayr D, Hiddemann W, Molls M, Multhoff G
Journal of translational medicine 2009 Jun 23;7:50
Journal of translational medicine 2009 Jun 23;7:50
Local immune alterations in oral mucosa of the edentulous patients; possible cause of teeth loss?
Zurac S, Girtan M, Lavric L, Petsakos G, Stăniceanu F, Bastian A, Andrei R, Popp C, Laba E
Romanian journal of internal medicine = Revue roumaine de medecine interne 2008;46(3):249-53
Romanian journal of internal medicine = Revue roumaine de medecine interne 2008;46(3):249-53
Homeostasis and effector function of lymphopenia-induced "memory-like" T cells in constitutively T cell-depleted mice.
Voehringer D, Liang HE, Locksley RM
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):4742-53
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):4742-53
BAFF-modulated repopulation of B lymphocytes in the blood and salivary glands of rituximab-treated patients with Sjögren's syndrome.
Pers JO, Devauchelle V, Daridon C, Bendaoud B, Le Berre R, Bordron A, Hutin P, Renaudineau Y, Dueymes M, Loisel S, Berthou C, Saraux A, Youinou P
Arthritis and rheumatism 2007 May;56(5):1464-77
Arthritis and rheumatism 2007 May;56(5):1464-77
CD8 tumor-infiltrating lymphocytes are predictive of survival in muscle-invasive urothelial carcinoma.
Sharma P, Shen Y, Wen S, Yamada S, Jungbluth AA, Gnjatic S, Bajorin DF, Reuter VE, Herr H, Old LJ, Sato E
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 6;104(10):3967-72
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 6;104(10):3967-72
Intraepithelial CD8+ tumor-infiltrating lymphocytes and a high CD8+/regulatory T cell ratio are associated with favorable prognosis in ovarian cancer.
Sato E, Olson SH, Ahn J, Bundy B, Nishikawa H, Qian F, Jungbluth AA, Frosina D, Gnjatic S, Ambrosone C, Kepner J, Odunsi T, Ritter G, Lele S, Chen YT, Ohtani H, Old LJ, Odunsi K
Proceedings of the National Academy of Sciences of the United States of America 2005 Dec 20;102(51):18538-43
Proceedings of the National Academy of Sciences of the United States of America 2005 Dec 20;102(51):18538-43
Immunohistochemical analysis of orbital connective tissue specimens of patients with active Graves ophthalmopathy.
Avunduk AM, Avunduk MC, Pazarli H, Oguz V, Varnell ED, Kaufman HE, Aksoy F
Current eye research 2005 Aug;30(8):631-8
Current eye research 2005 Aug;30(8):631-8
Etanercept treatment in the endotoxin-induced uveitis of rats.
Avunduk MC, Avunduk AM, Oztekin E, Baltaci AK, Ozyazgan Y, Mogolkoc R
Experimental eye research 2004 Sep;79(3):357-65
Experimental eye research 2004 Sep;79(3):357-65
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Supportive validation
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- Formalin-fixed, paraffin-embedded human tonsil stained with CD8 antibody using peroxidase-conjugate and DAB chromogen. Note membrane staining of T cells.
Supportive validation
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- Flow cytometry analysis of CD8 in THP-1 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD8 monoclonal antibody (Product # MA5-13473) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Fig. 2 Immuno-FISH and CD8+ T-cell infiltrate in primary vs. metastatic samples. a iFISH images of one area of the primary tumor and brain and liver metastases of case 15. Scale bar 100 mm. b Dot plot depicting relative frequencies of the four different cell types in primary and metastatic samples. Error bars, S.E.M. c Immunofluorescence analysis of CD8, GranzymeB (GZMB), and pSTAT3. Images are a montage of nine fields captured from one area of the tissue. Scale bar 100 mm. d , e Graph depicting numbers of CD8 + T cells per montage ( d ) and fraction of GZMB + CD8 + T cells ( e ). Significance of the difference between primary and metastatic samples was calculated using the Wilcoxon rank-sum test. f Correlation analysis between the number of infiltrating CD8 + T cells and the relative frequencies of specific cell populations in primary and metastatic samples. g Correlation analysis between number of infiltrating CD8 + T cells and the Shannon index of diversity in all, primary and metastatic samples. Gray area, 95% confidence interval. Sample sizes were n = 16 primary tumors and n = 18 metastases
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- Figure 1. Immunohistochemical analyses of intratumoral and peritumoral lymphocytes. Immunohistochemical staining of tumor-infiltrating (A) Foxp3 + T cells and (B) CD8 + T lymphocytes in invasive breast cancer. A diffuse pattern of intratumoral lymphocytes was observed. By contrast, peritumoral lymphocytes formed a lymphoid aggregate (x10 objective lens). There were markedly more Foxp3 + Tregs in the (C) peritumoral area than the (E) intratumoral areas. There was an increased number of CD8 + lymphocytes in the (D) peritumoral area than the (F) intratumoral areas. A and B, x10 objective lens; B-E, x40 objective lens. CD, cluster of differentiation; Foxp3, forkhead box 3.
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- Figure 3 UVR-induced inflammation recruits and maintains CD8 + GATA3 + T cells 14 days post-UVR . (a) Quantification of the total number of CD8 + cells on immunohistochemical staining ( N = 12 or 13 healthy volunteers for each time point). (b) Percentage of CD8 + GATA3 + cells of total CD8 + cells by immunofluorescence analysis ( N = 8 healthy volunteers for each time point). (c) Representative immunofluorescent images of CD8 + GATA3 + cells post-UVR. Data are mean +- SEM. Experiments were performed once. Scale bars = 50 um. * P < 0.05, ** P < 0.01, *** P < 0.001.
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- Fig. 3 Transcriptomic analysis demonstrates considerable ITH underlying convergent immune phenotypes. a Volcano plot of differentially expressed genes comparing high vs. low immune infiltrate regions across the tumor core and margins. Vertical red lines indicate a minimum twofold change in expression value; horizontal red line indicates the adjusted p -value threshold of
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- Figure 1 Matched haematoxylin & eosin (H&E) stained sections and multiplexed immunofluorescence (mIF) images from TMA cores of 2 patients tumours stained using Opal reagents: CD3 white, CD8 pink, CD20 orange, CD68 purple, PD-1 turquoise, PD-L1 yellow, FoxP3 green and Pan-CK red. DAPI nuclear counterstain is dark blue.. Patient 1 ( A - D ) & patient 2 ( E - H ) ( A , E : H&E x 200; B , F: mIF x 200; C and G: mIF x 400; D and H: H&E x 400).