Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB247-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-15 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-15 in Western blots. In Western blots, no cross-reactivity with recombinant human IL-2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P40933
- Isotype
- IgG
- Antibody clone number
- 34593
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
IL-15 expression on RA synovial fibroblasts promotes B cell survival.
Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15Rα.
TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Cloning and expression of feline interleukin 15.
Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
Regulatory activity of autocrine IL-10 on dendritic cell functions.
Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
IL-15 expression on RA synovial fibroblasts promotes B cell survival.
Benito-Miguel M, García-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibáñez T, Bonilla-Hernán MG, Pérez de Ayala C, Sánchez-Mateos P, Martín-Mola E, Miranda-Carús ME
PloS one 2012;7(7):e40620
PloS one 2012;7(7):e40620
Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15Rα.
Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
Blood 2011 Apr 14;117(15):4032-40
Blood 2011 Apr 14;117(15):4032-40
TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
Perrot I, Deauvieau F, Massacrier C, Hughes N, Garrone P, Durand I, Demaria O, Viaud N, Gauthier L, Blery M, Bonnefoy-Berard N, Morel Y, Tschopp J, Alexopoulou L, Trinchieri G, Paturel C, Caux C
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2080-8
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2080-8
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Cloning and expression of feline interleukin 15.
Dean GA, Barger A, Lavoy A
Cytokine 2005 Jan 21;29(2):77-83
Cytokine 2005 Jan 21;29(2):77-83
Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Münz C
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 23;101(47):16606-11
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 23;101(47):16606-11
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Miranda-Carús ME, Balsa A, Benito-Miguel M, Pérez de Ayala C, Martín-Mola E
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
Briard D, Brouty-Boyé D, Azzarone B, Jasmin C
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4326-32
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4326-32
Regulatory activity of autocrine IL-10 on dendritic cell functions.
Corinti S, Albanesi C, la Sala A, Pastore S, Girolomoni G
Journal of immunology (Baltimore, Md. : 1950) 2001 Apr 1;166(7):4312-8
Journal of immunology (Baltimore, Md. : 1950) 2001 Apr 1;166(7):4312-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-15 and Neutralization by Human IL-15 Antibody. Recombinant Human IL-15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-15 Monoclonal Antibody (Catalog # MAB247). The ND50 is typically 0.007-0.042 µg/mL.