AF1505

antibody from Novus Biologicals

Targeting: LRP6 ADCAD2

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF1505

Provider

Novus Biologicals

Product name

Goat Polyclonal LRP-6 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human LRP-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse LRP-6 is observed, and less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-4, and rhLRP-5 is observed.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

A novel high-content imaging-based technique for measuring binding of Dickkopf-1 to low-density lipoprotein receptor-related protein 6.

Priestley RS, Cheung J, Murphy EJ, Ehebauer MT, Davis JB, Di Daniel E
Journal of pharmacological and toxicological methods 2019 Jan - Feb;95:47-55

---

The Novel Secreted Adipokine WNT1-inducible Signaling Pathway Protein 2 (WISP2) Is a Mesenchymal Cell Activator of Canonical WNT.

Grünberg JR, Hammarstedt A, Hedjazifar S, Smith U
The Journal of biological chemistry 2014 Mar 7;289(10):6899-6907

---

Activation of canonical wingless-type MMTV integration site family (Wnt) signaling in mature adipocytes increases beta-catenin levels and leads to cell dedifferentiation and insulin resistance.

Gustafson B, Smith U
The Journal of biological chemistry 2010 Apr 30;285(18):14031-41

---

Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains.

Liu CC, Pearson C, Bu G
The Journal of biological chemistry 2009 May 29;284(22):15299-307

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human LRP-6 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with human LRP-6. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human LRP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1505) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LRP-6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.