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Western blot
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- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB120-13253 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-13253, RRID:AB_2175437
- Product name
- Rabbit Polyclonal Rab5a Antibody
- Antibody type
- Polyclonal
Submitted references LRRK2 inhibition prevents endolysosomal deficits seen in human Parkinson's disease.
Pharmacologically diverse antidepressants facilitate TRKB receptor activation by disrupting its interaction with the endocytic adaptor complex AP-2.
Mitochondrial ALDH2 protects against lipopolysaccharide-induced myocardial contractile dysfunction by suppression of ER stress and autophagy.
POT1b regulates phagocytosis and NO production by modulating activity of the small GTPase Rab5.
High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays.
Multiple regions contribute to membrane targeting of Rab GTPases.
The Rab GTPase family.
The Rab GTPase family.
Small GTP-binding proteins.
Rab proteins as membrane organizers.
Biogenesis of the sorting endosome: the role of Rab5.
Biogenesis of the sorting endosome: the role of Rab5.
Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11.
Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11.
Rocha EM, De Miranda BR, Castro S, Drolet R, Hatcher NG, Yao L, Smith SM, Keeney MT, Di Maio R, Kofler J, Hastings TG, Greenamyre JT
Neurobiology of disease 2020 Feb;134:104626
Neurobiology of disease 2020 Feb;134:104626
Pharmacologically diverse antidepressants facilitate TRKB receptor activation by disrupting its interaction with the endocytic adaptor complex AP-2.
Fred SM, Laukkanen L, Brunello CA, Vesa L, Göös H, Cardon I, Moliner R, Maritzen T, Varjosalo M, Casarotto PC, Castrén E
The Journal of biological chemistry 2019 Nov 29;294(48):18150-18161
The Journal of biological chemistry 2019 Nov 29;294(48):18150-18161
Mitochondrial ALDH2 protects against lipopolysaccharide-induced myocardial contractile dysfunction by suppression of ER stress and autophagy.
Pang J, Peng H, Wang S, Xu X, Xu F, Wang Q, Chen Y, Barton LA, Chen Y, Zhang Y, Ren J
Biochimica et biophysica acta. Molecular basis of disease 2019 Jun 1;1865(6):1627-1641
Biochimica et biophysica acta. Molecular basis of disease 2019 Jun 1;1865(6):1627-1641
POT1b regulates phagocytosis and NO production by modulating activity of the small GTPase Rab5.
Hagiwara M, Komatsu T, Sugiura SS, Isoda R, Tada H, Tanigawa N, Kato Y, Ishida N, Kobayashi K, Matsushita K
Biochemical and biophysical research communications 2013 Sep 27;439(3):413-7
Biochemical and biophysical research communications 2013 Sep 27;439(3):413-7
High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays.
Rho JH, Lampe PD
Journal of proteome research 2013 May 3;12(5):2311-20
Journal of proteome research 2013 May 3;12(5):2311-20
Multiple regions contribute to membrane targeting of Rab GTPases.
Ali BR, Wasmeier C, Lamoreux L, Strom M, Seabra MC
Journal of cell science 2004 Dec 15;117(Pt 26):6401-12
Journal of cell science 2004 Dec 15;117(Pt 26):6401-12
The Rab GTPase family.
Stenmark H, Olkkonen VM
Genome biology 2001;2(5):REVIEWS3007
Genome biology 2001;2(5):REVIEWS3007
The Rab GTPase family.
Stenmark H, Olkkonen VM
Genome biology 2001;2(5):REVIEWS3007
Genome biology 2001;2(5):REVIEWS3007
Small GTP-binding proteins.
Takai Y, Sasaki T, Matozaki T
Physiological reviews 2001 Jan;81(1):153-208
Physiological reviews 2001 Jan;81(1):153-208
Rab proteins as membrane organizers.
Zerial M, McBride H
Nature reviews. Molecular cell biology 2001 Feb;2(2):107-17
Nature reviews. Molecular cell biology 2001 Feb;2(2):107-17
Biogenesis of the sorting endosome: the role of Rab5.
Woodman PG
Traffic (Copenhagen, Denmark) 2000 Sep;1(9):695-701
Traffic (Copenhagen, Denmark) 2000 Sep;1(9):695-701
Biogenesis of the sorting endosome: the role of Rab5.
Woodman PG
Traffic (Copenhagen, Denmark) 2000 Sep;1(9):695-701
Traffic (Copenhagen, Denmark) 2000 Sep;1(9):695-701
Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11.
Sönnichsen B, De Renzis S, Nielsen E, Rietdorf J, Zerial M
The Journal of cell biology 2000 May 15;149(4):901-14
The Journal of cell biology 2000 May 15;149(4):901-14
Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11.
Sönnichsen B, De Renzis S, Nielsen E, Rietdorf J, Zerial M
The Journal of cell biology 2000 May 15;149(4):901-14
The Journal of cell biology 2000 May 15;149(4):901-14
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab5a Antibody [NB120-13253] - analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab5a Antibody [NB120-13253] - analysis using Rabbit Anti-Rab5 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
