Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [5]
- Other assay [2]
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Validation data
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- Product number
- STJ140060 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-RAB5A antibody (115aa C-Term) (STJ140060)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-RAB5A, member RAS oncogene family (115aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Handling
- NA
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Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence – anti-Rab5a antibody in transduced Hepa1-6 cells with Ad GFP-Rab5a at 1:50 dilution cells were fixed with 4% of PFA
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of mouse stomach using anti-Rab5a antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5a antibody at 1:250:DAB detection.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of mouse lung using anti-Rab5a antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5a antibody at 1:250:DAB detection.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of mouse liver using anti-Rab5a antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5a antibody at 1:250:DAB detection.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of mouse Intestine using anti-Rab5a antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5a antibody at 1:250:DAB detection.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Anti-Rab5a antibody at 1:500 dilution 293 cells transfected with GFP-Rab5 rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Anti-Rab5a antibody at 1:2500 dilution lysates at 50 µg per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA