LS-C407829

antibody from LSBio

Targeting: SFTPD COLEC7, SFTP4, SP-D

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C407829

Provider

LSBio

Product name

SFTPD / Surfactant Protein D Antibody (aa292-321) LS-C407829

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Rat

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Surfactant protein D antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Surfactant protein D antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Surfactant protein D antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.