MAB1920

antibody from R&D Systems

Targeting: SFTPD COLEC7, SFTP4, SP-D

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1920

Provider

R&D Systems

Product name

Human SP-D Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human SP-D in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P35247.2


Isotype

IgG

Antibody clone number

292201

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Fungal melanin stimulates surfactant protein D-mediated opsonization of and host immune response to Aspergillus fumigatus spores.

Wong SSW, Rani M, Dodagatta-Marri E, Ibrahim-Granet O, Kishore U, Bayry J, Latgé JP, Sahu A, Madan T, Aimanianda V
The Journal of biological chemistry 2018 Mar 30;293(13):4901-4912

ELISA

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Human SP-D ELISA Standard Curve. Recombinant Human SP-D protein was serially diluted 2-fold and captured by Mouse Anti-Human SP-D Monoclonal Antibody (Catalog # MAB19201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SP-D Monoclonal Antibody (Catalog # MAB1920) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).