sc-28305

antibody from Santa Cruz Biotechnology

Targeting: SP3 SPR-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28305

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28305, RRID:AB_628274

Product name

Anti-SP3

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

The tumor suppressor gene KCTD11REN is regulated by Sp1 and methylation and its expression is reduced in tumors.

Mancarelli MM, Zazzeroni F, Ciccocioppo L, Capece D, Po A, Murgo S, Di Camillo R, Rinaldi C, Ferretti E, Gulino A, Alesse E
Molecular cancer 2010 Jun 30;9:172

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Involvement of Sp1/Sp3 in the activation of the GATA-1 erythroid promoter in K562 cells

Chun Hui Hou, Jian Huang, Qi Ye He, Cai Ning Zhang, Xue Jun Zhang, Ruo Lan Qian
Cell Research 2008 Jan;18(2):302-310

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 82

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.