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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-33357 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33357, RRID:AB_10004066
- Product name
- Rabbit Polyclonal TRAF-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-6 Antibody [NBP1-33357] - TRAF6 antibody [N2C2], Internal detects TRAF6 protein by western blot analysis. A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 7.5 % SDS-PAGE TRAF6 antibody [N2C2], Internal ) dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-6 Antibody [NBP1-33357] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa C: HepG2 7.5% SDS PAGE diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-6 Antibody [NBP1-33357] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-6 Antibody [NBP1-33357] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 % SDS-PAGE. The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Paraffin-embedded 59T xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Mouse lung diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain antibody [N2C2], Internal diluted at 1:500.
