Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-1050-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Cytochrome C Antibody
- Antibody type
- Monoclonal
- Description
- Cytochrome C is an inner mitochondrial membrane hemeprotein encoded by CYCS gene. Cytochrome C has two major roles within the cell; firstly, it acts as an electron carrier between Complex III (Cytochrome bc1 complex) and Complex IV (Cytochrome C oxidase). The second important function of Cytochrome C is in apoptosis. In response to extrinsic or intrinsic signals and upon suppression of anti-apoptotic molecules, several pro-apoptotic molecules are activated that disturb the mitochondrial membrane permeability and causes the release of Cytochrome C. Cytochrome C then binds to apoptotic protease activating factor-1 (Apaf-1). This complex interacts with pro-caspase 9 and initiates the caspase cascade resulting in apoptosis. Human Cytochrome C has a molecular mass of 11 kDa. Defect in the CYCS gene is associated with autosomal dominant diseases like thrombocytopenia type 4 (THC4). High levels of Cytochrome C are found in blood leukocytes, kidney, liver, heart and brain.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Full length recombinant Cytochrome
C protein was used as the immunogen
for this antibody.- Isotype
- IgG
- Antibody clone number
- ABM30B3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Cytochrome C. Anti-Cytochrome C antibody (Clone: ABM30B3) was tested at 2 µg/ml on h Kidney lysate.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Cytochrome C. Anti-Cytochrome C antibody (Clone: ABM30B3) was tested at 2 µg/ml on (1) m Kidney, (2) r Kidney and (3) 3T3 lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular Flow analysis of Cytochrome C antibody in A431 cells using 0.5 µg/ 10^6 cells of anti-Cytochrome C antibody (ABM30B3). Green represents isotype control;red represents anti-Cytochrome C antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol