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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Western blot [2]
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- Product number
- MAB897 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Cytochrome c Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Reacts with denatured human, mouse and rat Cytochrome c, but not native Cytochrome c in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 7H8.2C12
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells.
Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming.
Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
HA14-1, a small molecule inhibitor of Bcl-2, bypasses chemoresistance in leukaemia cells.
Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
Xu L, Lin X, Zheng Y, Zhou H
Molecular medicine reports 2019 Jul;20(1):613-621
Molecular medicine reports 2019 Jul;20(1):613-621
Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming.
Sun S, Liu J, Zhao M, Han Y, Chen P, Mo Q, Wang B, Chen G, Fang Y, Tian Y, Zhou J, Ma D, Gao Q, Wu P
Cell death & disease 2017 Jun 8;8(6):e2870
Cell death & disease 2017 Jun 8;8(6):e2870
Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
Chávez-Galán L, Ramon-Luing LA, Torre-Bouscoulet L, Pérez-Padilla R, Sada-Ovalle I
PloS one 2013;8(11):e80971
PloS one 2013;8(11):e80971
HA14-1, a small molecule inhibitor of Bcl-2, bypasses chemoresistance in leukaemia cells.
Oliver L, Mahé B, Gréé R, Vallette FM, Juin P
Leukemia research 2007 Jun;31(6):859-63
Leukemia research 2007 Jun;31(6):859-63
Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
Experimental cell research 2004 Aug 15;298(2):560-73
Experimental cell research 2004 Aug 15;298(2):560-73
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Cytochrome c by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytochrome c at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cytochrome c by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
