Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX89740 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX89740, RRID:AB_10721581
- Product name
- GAPDH antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Drosophila
- Host
- Goat
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB analysis of HeLa lysate control and after si-RNA-mediated GAPDH knock-down (35£gg per lane) using GAPDH antibody at a concentration of 0.1 £gg/ml. Level of knock-down relative to Actin expression level was determined by RT-PCR.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX89740 staining (0.01?g/ml) of 293 lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells before (top) and after (bottom) si-RNA-mediated GAPDH knock-down using GAPDH antibody at a concentration of 0.1 ug/ml (green). Nuclei were stained wuth DAPI stain (blue).