ABIN1381745

antibody from antibodies-online

Targeting: MET DFNB97, HGFR, RCCP2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1381745

Provider

antibodies-online

Product name

anti-Met Proto-Oncogene (Hepatocyte Growth Factor Receptor) (MET) antibody

Antibody type

Monoclonal

Antigen

genetic immunisation with cDNA encoding human c-Met

Description

Protein G

Reactivity

Human

Isotype

IgG

Antibody clone number

BCI-3E7

Vial size

100 μg

Storage

short term: 2°C - 8°C, long term: -20°C

Handling

Avoid repeated freezing and thawing.

References

Submitted references

The met oncogene: from detection by transfection to transmembrane receptor for hepatocyte growth factor.

Cooper CS
Oncogene 1992 Jan;7(1):3-7

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Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product.

Bottaro DP, Rubin JS, Faletto DL, Chan AM, Kmiecik TE, Vande Woude GF, Aaronson SA
Science (New York, N.Y.) 1991 Feb 15;251(4995):802-4

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

SDS-PAGE analysis of purified BCI-3E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BCI-3C7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS analysis of BOSC23 cells using BCI-3E7. BOSC23 cells were transiently transfected with an expres-sion vector encoding either c-Met (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BCI-3E7 was detected with a PE-conjugated secondary antibody. A posi-tive signal was obtained only with c-Met transfected cells.