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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- MA5-32763 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nogo-B Recombinant Rabbit Monoclonal Antibody (JM02-34)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JM02-34
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nogo-B in mouse skeletal muscle tissue (1) and Hela cell (2) lysate using a Nogo-B Monoclonal antibody (Product # MA5-32763) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Reticulon-4 was achieved by transfecting U-87 MG with Reticulon-4 specific siRNAs (Silencer® select Product # s32768, s32766). Western Blot analysis (Fig. a) was performed using Membrane enriched extracts from the Reticulon-4 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2), and untransfected cells (lane 1). The Blot was probed with Nogo-B Recombinant Rabbit Monoclonal Antibody (JM02-34) (Product # MA5-32763, 1:1,000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western Blot is shown in histogram (Fig. b). The decrease in signal upon siRNA mediated knockdown confirms that the antibody is specific to Reticulon-4.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Nogo-B Recombinant Rabbit Monoclonal Antibody (JM02-34) (Product # MA5-32763) and 40-50 kDa bands corresponding to Reticulon-4 was observed across all the cell lines and tissue lysates tested. An ~200 kDa band was also observed in HUVEC cell line that corresponds to Nogo- A. Membrane enriched extracts (30 µg lysate) of HUVEC (Lane 1), HAEC (Lane 2), BJ (Lane 3), Hep G2 (Lane 4), Neuro-2a (Lane 5), C2C12 (Lane 6), THP-1 (Lane 7) as well as Mouse Prostate (Lane 8) and Mouse Liver tissue lysates (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Nogo-B in A549 cells using a Nogo-B Monoclonal antibody (Product # MA5-32763) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Nogo-B in HepG2 cells using a Nogo-B Monoclonal antibody (Product # MA5-32763) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Nogo-B in SH-SY5Y cells using a Nogo-B Monoclonal antibody (Product # MA5-32763) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Nogo-B of paraffin-embedded Human breast tissue using a Nogo-B Monoclonal antibody (Product #MA5-32763). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Nogo-B of paraffin-embedded Human fetal skeletal muscle tissue using a Nogo-B Monoclonal antibody (Product #MA5-32763). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Nogo-B of paraffin-embedded Mouse testis tissue using a Nogo-B Monoclonal antibody (Product #MA5-32763). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Nogo-B of paraffin-embedded Mouse brain tissue using a Nogo-B Monoclonal antibody (Product #MA5-32763). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of Nogo-B in Hela cells using a Nogo-B Monoclonal Antibody (Product # MA5-32763) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).
