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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-367 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-367, RRID:AB_2194226
- Product name
- Anti-SREBF1
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Blockade of interleukin-6 signaling enhances hepatic steatosis but improves liver injury in methionine choline-deficient diet-fed mice
Myocardial lipid accumulation in patients with pressure-overloaded heart and metabolic syndrome.
A role for PPARalpha in the control of SREBP activity and lipid synthesis in the liver.
Kanji Yamaguchi, Yoshito Itoh, Chihiro Yokomizo, Takeshi Nishimura, Toshihisa Niimi, Hideki Fujii, Takeshi Okanoue, Toshikazu Yoshikawa
Laboratory Investigation 2010 Apr;90(8):1169-1178
Laboratory Investigation 2010 Apr;90(8):1169-1178
Myocardial lipid accumulation in patients with pressure-overloaded heart and metabolic syndrome.
Marfella R, Di Filippo C, Portoghese M, Barbieri M, Ferraraccio F, Siniscalchi M, Cacciapuoti F, Rossi F, D'Amico M, Paolisso G
Journal of lipid research 2009 Nov;50(11):2314-23
Journal of lipid research 2009 Nov;50(11):2314-23
A role for PPARalpha in the control of SREBP activity and lipid synthesis in the liver.
Knight BL, Hebbachi A, Hauton D, Brown AM, Wiggins D, Patel DD, Gibbons GF
The Biochemical journal 2005 Jul 15;389(Pt 2):413-21
The Biochemical journal 2005 Jul 15;389(Pt 2):413-21
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 76
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
