PAB10221

antibody from Abnova Corporation

Targeting: AP1G1 ADTG, CLAPG1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10221

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10221, RRID:AB_1671684

Product name

AP1G1 polyclonal antibody

Antibody type

Polyclonal

Description

Goat polyclonal antibody raised against synthetic peptide of AP1G1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Gamma-adaptin interacts directly with Rabaptin-5 through its ear domain.

Shiba Y, Takatsu H, Shin HW, Nakayama K
Journal of biochemistry 2002 Mar;131(3):327-36

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Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain.

Nogi T, Shiba Y, Kawasaki M, Shiba T, Matsugaki N, Igarashi N, Suzuki M, Kato R, Takatsu H, Nakayama K, Wakatsuki S
Nature structural biology 2002 Jul;9(7):527-31

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Identification and characterization of novel clathrin adaptor-related proteins.

Takatsu H, Sakurai M, Shin HW, Murakami K, Nakayama K
The Journal of biological chemistry 1998 Sep 18;273(38):24693-700

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using AP1G1 polyclonal antibody (Cat # PAB10221) shows strong detection of a 91 KDa band corresponding to human AP1G1 in a HeLa whole cell lysate (Lane 1).Peptide competition (using 1 ug/mL of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (Lane 2).Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of AP1G1 polyclonal antibody.Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.A chemiluminescence system was used for signal detection using a 60-sec exposure time.