Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Flow cytometry [4]
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Validation data
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- Product number
- NBP2-52437 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal RHAMM/CD168 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.
Shea DJ, Li YW, Stebe KJ, Konstantopoulos K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Nov;31(11):5078-5086
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Nov;31(11):5078-5086
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against human CD168 (AA: 306-497) recombinant protein. (Expected MW is 48.3 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against HEK293 (1) and CD168 (AA: 306-497)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis of Hela cells using CD168 mouse mAb (green) and negative control (red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437AF488 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on A549 cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.