Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-31870 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RHAMM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human ESC.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-RHAMM Polyclonal Antibody (Product # PA5-31870) and a 82 kDa band corresponding to Hyaluronan mediated motility receptor was observed across the cell lines tested with a uncharacterized non specific band (*). Whole cell extracts (30 µg lysate) of Reh (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HeLa (Lane 4), HEL (Lane 5), T-47D (Lane 6), MCF7 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RHAMM Polyclonal Antibody (Product # PA5-31870). Sample (20 µg of whole cell lysate). Lane A: human ESC . 7.5% SDS PAGE. RHAMM Polyclonal Antibody (Product # PA5-31870) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using RHAMM (Product # PA5-31870) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RHAMM antibody [N1N2], N-term detects RHAMM protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RHAMM antibody [N1N2], N-term (Product # PA5-31870) dilution: 1:250. Scale bar = 100 µm. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.