Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 648901 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#648901, RRID:AB_2235533
- Product name
- Purified anti-MyD88
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide (c-terminus)
- Description
- MyD88, originally isolated as myeloid differentiation primary response gene, is expressed in a variety of tissues. MyD88 serves as a cytoplasmic adaptor protein involved in the signaling of TLR and IL-1R family members. It associates with and recruits IRAK to IL-1R complex in response to IL-1. This pathway leads to activation of NF-kB. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18. MyD88 deficient mice lack a response to LPS (a bacterial product) which employs toll like receptors 2 and 4 (TLR2 and TLR4) as signaling receptors.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 25 µl
- Storage
- The antibody solution should be stored undiluted at -20°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Raji whole cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with a rabbit anti-MYD88 polyclonal antibody (Poly6489). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluniscence system.
- Conjugate
- Unconjugated