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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [3]
- Other assay [1]
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- Product number
- MA5-16231 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MyD88 Monoclonal Antibody (4D6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D6
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Recognition of Double-Stranded RNA and Regulation of Interferon Pathway by Toll-Like Receptor 10.
Association between toll-like receptor 8 expression and adverse clinical outcomes in patients with enterovirus-associated dilated cardiomyopathy.
Lee SM, Yip TF, Yan S, Jin DY, Wei HL, Guo RT, Peiris JSM
Frontiers in immunology 2018;9:516
Frontiers in immunology 2018;9:516
Association between toll-like receptor 8 expression and adverse clinical outcomes in patients with enterovirus-associated dilated cardiomyopathy.
Satoh M, Akatsu T, Ishikawa Y, Minami Y, Takahashi Y, Nakamura M
American heart journal 2007 Sep;154(3):581-8
American heart journal 2007 Sep;154(3):581-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MyD88 in A) human ovary, B) human prostate and Jurkat cell lysate in the C) absence and D) presence of immunizing peptide using a MyD88 monoclonal antibody (Product # MA5-16231) at 5 µg/mL. Goat anti-mouse Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MyD88 in A) human ovary, B) human prostate and Jurkat cell lysate in the C) absence and D) presence of immunizing peptide. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) followed by a goat anti-mouse Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MyD88 in MCF-7 cells. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 5 µg/mL. Isotype control (top) and MyD88 Antibody (bottom).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in MCF7 cells (blue) and a matched isotype control (orange). Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in Jurkat cells. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 0.5 µg followed by anti-mouse IgG PE conjugated secondary antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in Jurkat cells. Samples were incubated in MyD88 monoclonal (Product # MA5-16231) using a dilution of 10 µl (0.5 µg). Green represents isotype control ; red represents anti-MyD88 antibody (conjugated to Alexa Fluor® 488).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
