Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-81527 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NR3C2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of NR3C2 in Lane A: MCF7 Whole Cell Lysate (30 µg). Samples were probed using a NR3C2 Polyclonal Antibody (Product # PA5-81527) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:107 kDa. Observed band size:107 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NR3C2 Polyclonal Antibody (Product # PA5-81527) at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of NR3C2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NR3C2 Polyclonal Antibody (Product # PA5-81527, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NR3C2 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 4 µL with NR3C2 Polyclonal Antibody (Product # PA5-81527) and 15 µL of 50 % Protein G agarose. Primary antibody: NR3C2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa.