NB110-58358

antibody from Novus Biologicals

Targeting: YAP1 YAP65

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB110-58358

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-58358, RRID:AB_922796

Product name

Rabbit Polyclonal YAP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Expected reactivity based on immunogen homology: Isoform 4 (100%), Isoform 6 (100%)

Reactivity

Human, Mouse, Drosophila

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Metformin and LW6 impairs pancreatic cancer cells and reduces nuclear localization of YAP1.

Zhang X, Liu P, Shang Y, Kerndl H, Kumstel S, Gong P, Vollmar B, Zechner D
Journal of Cancer 2020;11(2):479-487

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Initiation of human mammary cell tumorigenesis by mutant KRAS requires YAP inactivation.

Lefort S, Tan S, Balani S, Rafn B, Pellacani D, Hirst M, Sorensen PH, Eaves CJ
Oncogene 2020 Feb;39(9):1957-1968

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The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts.

Liu T, Zhou L, Yang K, Iwasawa K, Kadekaro AL, Takebe T, Andl T, Zhang Y
Signal transduction and targeted therapy 2019;4:63

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Targeting plasma membrane phosphatidylserine content to inhibit oncogenic KRAS function.

Kattan WE, Chen W, Ma X, Lan TH, van der Hoeven D, van der Hoeven R, Hancock JF
Life science alliance 2019 Oct;2(5)

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The Hippo Pathway Blocks Mammalian Retinal Müller Glial Cell Reprogramming.

Rueda EM, Hall BM, Hill MC, Swinton PG, Tong X, Martin JF, Poché RA
Cell reports 2019 May 7;27(6):1637-1649.e6

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The Myb-MuvB Complex Is Required for YAP-Dependent Transcription of Mitotic Genes.

Pattschull G, Walz S, Gründl M, Schwab M, Rühl E, Baluapuri A, Cindric-Vranesic A, Kneitz S, Wolf E, Ade CP, Rosenwald A, von Eyss B, Gaubatz S
Cell reports 2019 Jun 18;27(12):3533-3546.e7

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Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma.

Chen P, Zhao D, Li J, Liang X, Li J, Chang A, Henry VK, Lan Z, Spring DJ, Rao G, Wang YA, DePinho RA
Cancer cell 2019 Jun 10;35(6):868-884.e6

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Network-based expression analyses and experimental validations revealed high co-expression between Yap1 and stem cell markers compared to differentiated cells.

Dehghanian F, Hojati Z, Esmaeili F, Masoudi-Nejad A
Genomics 2019 Jul;111(4):831-839

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Activated YAP causes renal damage of type 2 diabetic nephropathy.

Ma R, Ren JM, Li P, Zhou YJ, Zhou MK, Hu Z, Xiao XY
European review for medical and pharmacological sciences 2019 Jan;23(2):755-763

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Deficiency of Yes-Associated Protein Induces Cataract in Mice.

He Q, Gao Y, Wang T, Zhou L, Zhou W, Yuan Z
Aging and disease 2019 Apr;10(2):293-306

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A RhoA-YAP-c-Myc signaling axis promotes the development of polycystic kidney disease.

Cai J, Song X, Wang W, Watnick T, Pei Y, Qian F, Pan D
Genes & development 2018 Jun 1;32(11-12):781-793

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Hippo Signaling Pathway Dysregulation in Human Huntington's Disease Brain and Neuronal Stem Cells.

Mueller KA, Glajch KE, Huizenga MN, Wilson RA, Granucci EJ, Dios AM, Tousley AR, Iuliano M, Weisman E, LaQuaglia MJ, DiFiglia M, Kegel-Gleason K, Vakili K, Sadri-Vakili G
Scientific reports 2018 Jul 27;8(1):11355

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Ajuba inhibits hepatocellular carcinoma cell growth via targeting of β-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation.

Liu M, Jiang K, Lin G, Liu P, Yan Y, Ye T, Yao G, Barr MP, Liang D, Wang Y, Gong P, Meng S, Piao H
Journal of experimental & clinical cancer research : CR 2018 Jul 24;37(1):165

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Endocardial Hippo signaling regulates myocardial growth and cardiogenesis.

Artap S, Manderfield LJ, Smith CL, Poleshko A, Aghajanian H, See K, Li L, Jain R, Epstein JA
Developmental biology 2018 Aug 1;440(1):22-30

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Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development.

Xiao Y, Hill MC, Zhang M, Martin TJ, Morikawa Y, Wang S, Moise AR, Wythe JD, Martin JF
Developmental cell 2018 Apr 23;45(2):153-169.e6

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Hippo pathway deficiency reverses systolic heart failure after infarction.

Leach JP, Heallen T, Zhang M, Rahmani M, Morikawa Y, Hill MC, Segura A, Willerson JT, Martin JF
Nature 2017 Oct 12;550(7675):260-264

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Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue.

Plubell DL, Wilmarth PA, Zhao Y, Fenton AM, Minnier J, Reddy AP, Klimek J, Yang X, David LL, Pamir N
Molecular & cellular proteomics : MCP 2017 May;16(5):873-890

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Deubiquitinase YOD1 potentiates YAP/TAZ activities through enhancing ITCH stability.

Kim Y, Kim W, Song Y, Kim JR, Cho K, Moon H, Ro SW, Seo E, Ryu YM, Myung SJ, Jho EH
Proceedings of the National Academy of Sciences of the United States of America 2017 May 2;114(18):4691-4696

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YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis.

Lin C, Yao E, Zhang K, Jiang X, Croll S, Thompson-Peer K, Chuang PT
eLife 2017 Mar 21;6

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PRKCI promotes immune suppression in ovarian cancer.

Sarkar S, Bristow CA, Dey P, Rai K, Perets R, Ramirez-Cardenas A, Malasi S, Huang-Hobbs E, Haemmerle M, Wu SY, McGuire M, Protopopov A, Jiang S, Liu JF, Hirsch MS, Chang Q, Lazar AJ, Sood AK, Drapkin R, DePinho R, Draetta G, Chin L
Genes & development 2017 Jun 1;31(11):1109-1121

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Dystrophin-glycoprotein complex sequesters Yap to inhibit cardiomyocyte proliferation.

Morikawa Y, Heallen T, Leach J, Xiao Y, Martin JF
Nature 2017 Jul 13;547(7662):227-231

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The extracellular matrix protein agrin promotes heart regeneration in mice.

Bassat E, Mutlak YE, Genzelinakh A, Shadrin IY, Baruch Umansky K, Yifa O, Kain D, Rajchman D, Leach J, Riabov Bassat D, Udi Y, Sarig R, Sagi I, Martin JF, Bursac N, Cohen S, Tzahor E
Nature 2017 Jul 13;547(7662):179-184

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The Hippo pathway regulator KIBRA promotes podocyte injury by inhibiting YAP signaling and disrupting actin cytoskeletal dynamics.

Meliambro K, Wong JS, Ray J, Calizo RC, Towne S, Cole B, El Salem F, Gordon RE, Kaufman L, He JC, Azeloglu EU, Campbell KN
The Journal of biological chemistry 2017 Dec 22;292(51):21137-21148

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Homeostatic Control of Hpo/MST Kinase Activity through Autophosphorylation-Dependent Recruitment of the STRIPAK PP2A Phosphatase Complex.

Zheng Y, Liu B, Wang L, Lei H, Pulgar Prieto KD, Pan D
Cell reports 2017 Dec 19;21(12):3612-3623

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Actomyosin contractility provokes contact inhibition in E-cadherin-ligated keratinocytes.

Hirata H, Samsonov M, Sokabe M
Scientific reports 2017 Apr 13;7:46326

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MicroRNA Regulates Hepatocytic Differentiation of Progenitor Cells by Targeting YAP1.

Jung KH, McCarthy RL, Zhou C, Uprety N, Barton MC, Beretta L
Stem cells (Dayton, Ohio) 2016 May;34(5):1284-96

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Pitx2 promotes heart repair by activating the antioxidant response after cardiac injury.

Tao G, Kahr PC, Morikawa Y, Zhang M, Rahmani M, Heallen TR, Li L, Sun Z, Olson EN, Amendt BA, Martin JF
Nature 2016 Jun 2;534(7605):119-23

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A Hippo and Fibroblast Growth Factor Receptor Autocrine Pathway in Cholangiocarcinoma.

Rizvi S, Yamada D, Hirsova P, Bronk SF, Werneburg NW, Krishnan A, Salim W, Zhang L, Trushina E, Truty MJ, Gores GJ
The Journal of biological chemistry 2016 Apr 8;291(15):8031-47

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Matrix-Stiffness-Regulated Inverse Expression of Krüppel-Like Factor 5 and Krüppel-Like Factor 4 in the Pathogenesis of Renal Fibrosis.

Chen WC, Lin HH, Tang MJ
The American journal of pathology 2015 Sep;185(9):2468-81

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RUNX2 and TAZ-dependent signaling pathways regulate soluble E-Cadherin levels and tumorsphere formation in breast cancer cells.

Brusgard JL, Choe M, Chumsri S, Renoud K, MacKerell AD Jr, Sudol M, Passaniti A
Oncotarget 2015 Sep 29;6(29):28132-50

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Yes-associated protein (YAP) is a negative regulator of chondrogenesis in mesenchymal stem cells.

Karystinou A, Roelofs AJ, Neve A, Cantatore FP, Wackerhage H, De Bari C
Arthritis research & therapy 2015 May 30;17(1):147

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Homeostatic control of Hippo signaling activity revealed by an endogenous activating mutation in YAP.

Chen Q, Zhang N, Xie R, Wang W, Cai J, Choi KS, David KK, Huang B, Yabuta N, Nojima H, Anders RA, Pan D
Genes & development 2015 Jun 15;29(12):1285-97

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β-Catenin destruction complex-independent regulation of Hippo-YAP signaling by APC in intestinal tumorigenesis.

Cai J, Maitra A, Anders RA, Taketo MM, Pan D
Genes & development 2015 Jul 15;29(14):1493-506

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Yes-associated protein (YAP) modulates oncogenic features and radiation sensitivity in endometrial cancer.

Tsujiura M, Mazack V, Sudol M, Kaspar HG, Nash J, Carey DJ, Gogoi R
PloS one 2014;9(6):e100974

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Yap1 activation enables bypass of oncogenic Kras addiction in pancreatic cancer.

Kapoor A, Yao W, Ying H, Hua S, Liewen A, Wang Q, Zhong Y, Wu CJ, Sadanandam A, Hu B, Chang Q, Chu GC, Al-Khalil R, Jiang S, Xia H, Fletcher-Sananikone E, Lim C, Horwitz GI, Viale A, Pettazzoni P, Sanchez N, Wang H, Protopopov A, Zhang J, Heffernan T, Johnson RL, Chin L, Wang YA, Draetta G, DePinho RA
Cell 2014 Jul 3;158(1):185-197

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Knockdown of GPC3 inhibits the proliferation of Huh7 hepatocellular carcinoma cells through down-regulation of YAP.

Miao HL, Pan ZJ, Lei CJ, Wen JY, Li MY, Liu ZK, Qiu ZD, Lin MZ, Chen NP, Chen M
Journal of cellular biochemistry 2013 Mar;114(3):625-31

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and actomyosin activity on YAP phosphorylation. HaCaT cells cultured for 40 h under the sparse and confluent conditions were treated with 100 uM blebbistatin (Blebb) or DMSO (for control) for 6 h, and then lysed and immunoblotted for Ser127-phosphorylated YAP (pYAP), YAP, beta-catenin and actin. Similar results were obtained in two independent experiments. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep46326), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: YAP1 Antibody [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells transfected with empty vector control (pLKO.1) were harvested, and 20 ug was subjected to SDS-PAGE and used for Western blotting. Image collected and cropped by CiteAb from the following publication (http://www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900431), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at 1:200 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.