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- References [34]
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- Product number
- H00010413-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010413-M01, RRID:AB_549187
- Product name
- Mouse Monoclonal YAP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. Expected cross reactivity based on immunogen sequence isoforms: Isoform 1 (100%), Isoform 2 (100%), Isoform 3 (100%), Isoform 6 (100)%, Isoform 7 (100%), Isoform 8 (100%), Isoform 9 (100%)
- Reactivity
- Human, Mouse, Canine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The CalcR-PKA-Yap1 Axis Is Critical for Maintaining Quiescence in Muscle Stem Cells.
RHOA activity in expanding blastocysts is essential to regulate HIPPO-YAP signaling and to maintain the trophectoderm-specific gene expression program in a ROCK/actin filament-independent manner.
Citron kinase interacts with LATS2 and inhibits its activity by occluding its hydrophobic phosphorylation motif.
Regulation of Sox2 and stemness by nicotine and electronic-cigarettes in non-small cell lung cancer.
Yap/Taz transcriptional activity is essential for vascular regression via Ctgf expression and actin polymerization.
Mechanical cue-induced YAP instructs Skp2-dependent cell cycle exit and oncogenic signaling.
Toxin A of the nosocomial pathogen Clostridium difficile induces primary effects in the proteome of HEp-2 cells.
Overexpression of TEAD4 in atypical teratoid/rhabdoid tumor: New insight to the pathophysiology of an aggressive brain tumor.
The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo.
Common and Distinctive Functions of the Hippo Effectors Taz and Yap in Skeletal Muscle Stem Cell Function.
The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin.
Multiple Mechanisms Cooperate to Constitutively Exclude the Transcriptional Co-Activator YAP from the Nucleus During Murine Oogenesis.
Statins inhibit blastocyst formation by preventing geranylgeranylation.
Early preimplantation cells expressing Cdx2 exhibit plasticity of specification to TE and ICM lineages through positional changes.
Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition.
Asymmetric division of contractile domains couples cell positioning and fate specification.
Par-aPKC-dependent and -independent mechanisms cooperatively control cell polarity, Hippo signaling, and cell positioning in 16-cell stage mouse embryos.
Active YAP promotes pancreatic cancer cell motility, invasion and tumorigenesis in a mitotic phosphorylation-dependent manner through LPAR3.
Oncoprotein YAP regulates the spindle checkpoint activation in a mitotic phosphorylation-dependent manner through up-regulation of BubR1.
A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response.
Yap tunes airway epithelial size and architecture by regulating the identity, maintenance, and self-renewal of stem cells.
Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.
Serum deprivation inhibits the transcriptional co-activator YAP and cell growth via phosphorylation of the 130-kDa isoform of Angiomotin by the LATS1/2 protein kinases.
CDK1 phosphorylation of YAP promotes mitotic defects and cell motility and is essential for neoplastic transformation.
Amot130 adapts atrophin-1 interacting protein 4 to inhibit yes-associated protein signaling and cell growth.
SOX2 regulates YAP1 to maintain stemness and determine cell fate in the osteo-adipo lineage.
YAP1 recruits c-Abl to protect angiomotin-like 1 from Nedd4-mediated degradation.
Tead4 is constitutively nuclear, while nuclear vs. cytoplasmic Yap distribution is regulated in preimplantation mouse embryos.
Hippo pathway regulation by cell morphology and stress fibers.
Inactivation of Merlin in malignant mesothelioma cells and the Hippo signaling cascade dysregulation.
Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact.
LATS2 is a tumor suppressor gene of malignant mesothelioma.
JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis.
Merlin is a potent inhibitor of glioma growth.
Zhang L, Noguchi YT, Nakayama H, Kaji T, Tsujikawa K, Ikemoto-Uezumi M, Uezumi A, Okada Y, Doi T, Watanabe S, Braun T, Fujio Y, Fukada SI
Cell reports 2019 Nov 19;29(8):2154-2163.e5
Cell reports 2019 Nov 19;29(8):2154-2163.e5
RHOA activity in expanding blastocysts is essential to regulate HIPPO-YAP signaling and to maintain the trophectoderm-specific gene expression program in a ROCK/actin filament-independent manner.
Marikawa Y, Alarcon VB
Molecular human reproduction 2019 Feb 1;25(2):43-60
Molecular human reproduction 2019 Feb 1;25(2):43-60
Citron kinase interacts with LATS2 and inhibits its activity by occluding its hydrophobic phosphorylation motif.
Tran THY, Yang DW, Kim M, Lee DH, Gai M, Di Cunto F, Choi KW, Lim DS
Journal of molecular cell biology 2019 Dec 23;11(11):1006-1017
Journal of molecular cell biology 2019 Dec 23;11(11):1006-1017
Regulation of Sox2 and stemness by nicotine and electronic-cigarettes in non-small cell lung cancer.
Schaal CM, Bora-Singhal N, Kumar DM, Chellappan SP
Molecular cancer 2018 Oct 15;17(1):149
Molecular cancer 2018 Oct 15;17(1):149
Yap/Taz transcriptional activity is essential for vascular regression via Ctgf expression and actin polymerization.
Nagasawa-Masuda A, Terai K
PloS one 2017;12(4):e0174633
PloS one 2017;12(4):e0174633
Mechanical cue-induced YAP instructs Skp2-dependent cell cycle exit and oncogenic signaling.
Jang W, Kim T, Koo JS, Kim SK, Lim DS
The EMBO journal 2017 Sep 1;36(17):2510-2528
The EMBO journal 2017 Sep 1;36(17):2510-2528
Toxin A of the nosocomial pathogen Clostridium difficile induces primary effects in the proteome of HEp-2 cells.
Junemann J, Birgin G, Erdmann J, Schröder A, Just I, Gerhard R, Pich A
Proteomics. Clinical applications 2017 Mar;11(3-4)
Proteomics. Clinical applications 2017 Mar;11(3-4)
Overexpression of TEAD4 in atypical teratoid/rhabdoid tumor: New insight to the pathophysiology of an aggressive brain tumor.
Suzuki M, Kondo A, Ogino I, Arai H, Tomita T, Sredni ST
Pediatric blood & cancer 2017 Jul;64(7)
Pediatric blood & cancer 2017 Jul;64(7)
The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo.
Korotkevich E, Niwayama R, Courtois A, Friese S, Berger N, Buchholz F, Hiiragi T
Developmental cell 2017 Feb 6;40(3):235-247.e7
Developmental cell 2017 Feb 6;40(3):235-247.e7
Common and Distinctive Functions of the Hippo Effectors Taz and Yap in Skeletal Muscle Stem Cell Function.
Sun C, De Mello V, Mohamed A, Ortuste Quiroga HP, Garcia-Munoz A, Al Bloshi A, Tremblay AM, von Kriegsheim A, Collie-Duguid E, Vargesson N, Matallanas D, Wackerhage H, Zammit PS
Stem cells (Dayton, Ohio) 2017 Aug;35(8):1958-1972
Stem cells (Dayton, Ohio) 2017 Aug;35(8):1958-1972
The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin.
Furukawa KT, Yamashita K, Sakurai N, Ohno S
Cell reports 2017 Aug 8;20(6):1435-1447
Cell reports 2017 Aug 8;20(6):1435-1447
Multiple Mechanisms Cooperate to Constitutively Exclude the Transcriptional Co-Activator YAP from the Nucleus During Murine Oogenesis.
Abbassi L, Malki S, Cockburn K, Macaulay A, Robert C, Rossant J, Clarke HJ
Biology of reproduction 2016 May;94(5):102
Biology of reproduction 2016 May;94(5):102
Statins inhibit blastocyst formation by preventing geranylgeranylation.
Alarcon VB, Marikawa Y
Molecular human reproduction 2016 May;22(5):350-63
Molecular human reproduction 2016 May;22(5):350-63
Early preimplantation cells expressing Cdx2 exhibit plasticity of specification to TE and ICM lineages through positional changes.
Toyooka Y, Oka S, Fujimori T
Developmental biology 2016 Mar 1;411(1):50-60
Developmental biology 2016 Mar 1;411(1):50-60
Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition.
Kim M, Kim M, Park SJ, Lee C, Lim DS
EMBO reports 2016 Jan;17(1):64-78
EMBO reports 2016 Jan;17(1):64-78
Asymmetric division of contractile domains couples cell positioning and fate specification.
Maître JL, Turlier H, Illukkumbura R, Eismann B, Niwayama R, Nédélec F, Hiiragi T
Nature 2016 Aug 18;536(7616):344-348
Nature 2016 Aug 18;536(7616):344-348
Par-aPKC-dependent and -independent mechanisms cooperatively control cell polarity, Hippo signaling, and cell positioning in 16-cell stage mouse embryos.
Hirate Y, Hirahara S, Inoue K, Kiyonari H, Niwa H, Sasaki H
Development, growth & differentiation 2015 Oct;57(8):544-56
Development, growth & differentiation 2015 Oct;57(8):544-56
Active YAP promotes pancreatic cancer cell motility, invasion and tumorigenesis in a mitotic phosphorylation-dependent manner through LPAR3.
Yang S, Zhang L, Purohit V, Shukla SK, Chen X, Yu F, Fu K, Chen Y, Solheim J, Singh PK, Song W, Dong J
Oncotarget 2015 Nov 3;6(34):36019-31
Oncotarget 2015 Nov 3;6(34):36019-31
Oncoprotein YAP regulates the spindle checkpoint activation in a mitotic phosphorylation-dependent manner through up-regulation of BubR1.
Yang S, Zhang L, Chen X, Chen Y, Dong J
The Journal of biological chemistry 2015 Mar 6;290(10):6191-202
The Journal of biological chemistry 2015 Mar 6;290(10):6191-202
A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response.
Shao D, Zhai P, Del Re DP, Sciarretta S, Yabuta N, Nojima H, Lim DS, Pan D, Sadoshima J
Nature communications 2014;5:3315
Nature communications 2014;5:3315
Yap tunes airway epithelial size and architecture by regulating the identity, maintenance, and self-renewal of stem cells.
Zhao R, Fallon TR, Saladi SV, Pardo-Saganta A, Villoria J, Mou H, Vinarsky V, Gonzalez-Celeiro M, Nunna N, Hariri LP, Camargo F, Ellisen LW, Rajagopal J
Developmental cell 2014 Jul 28;30(2):151-65
Developmental cell 2014 Jul 28;30(2):151-65
Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.
Das A, Tanigawa S, Karner CM, Xin M, Lum L, Chen C, Olson EN, Perantoni AO, Carroll TJ
Nature cell biology 2013 Sep;15(9):1035-44
Nature cell biology 2013 Sep;15(9):1035-44
Serum deprivation inhibits the transcriptional co-activator YAP and cell growth via phosphorylation of the 130-kDa isoform of Angiomotin by the LATS1/2 protein kinases.
Adler JJ, Johnson DE, Heller BL, Bringman LR, Ranahan WP, Conwell MD, Sun Y, Hudmon A, Wells CD
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 22;110(43):17368-73
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 22;110(43):17368-73
CDK1 phosphorylation of YAP promotes mitotic defects and cell motility and is essential for neoplastic transformation.
Yang S, Zhang L, Liu M, Chong R, Ding SJ, Chen Y, Dong J
Cancer research 2013 Nov 15;73(22):6722-33
Cancer research 2013 Nov 15;73(22):6722-33
Amot130 adapts atrophin-1 interacting protein 4 to inhibit yes-associated protein signaling and cell growth.
Adler JJ, Heller BL, Bringman LR, Ranahan WP, Cocklin RR, Goebl MG, Oh M, Lim HS, Ingham RJ, Wells CD
The Journal of biological chemistry 2013 May 24;288(21):15181-93
The Journal of biological chemistry 2013 May 24;288(21):15181-93
SOX2 regulates YAP1 to maintain stemness and determine cell fate in the osteo-adipo lineage.
Seo E, Basu-Roy U, Gunaratne PH, Coarfa C, Lim DS, Basilico C, Mansukhani A
Cell reports 2013 Jun 27;3(6):2075-87
Cell reports 2013 Jun 27;3(6):2075-87
YAP1 recruits c-Abl to protect angiomotin-like 1 from Nedd4-mediated degradation.
Skouloudaki K, Walz G
PloS one 2012;7(4):e35735
PloS one 2012;7(4):e35735
Tead4 is constitutively nuclear, while nuclear vs. cytoplasmic Yap distribution is regulated in preimplantation mouse embryos.
Hirate Y, Cockburn K, Rossant J, Sasaki H
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 11;109(50):E3389-90; author reply E3391-2
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 11;109(50):E3389-90; author reply E3391-2
Hippo pathway regulation by cell morphology and stress fibers.
Wada K, Itoga K, Okano T, Yonemura S, Sasaki H
Development (Cambridge, England) 2011 Sep;138(18):3907-14
Development (Cambridge, England) 2011 Sep;138(18):3907-14
Inactivation of Merlin in malignant mesothelioma cells and the Hippo signaling cascade dysregulation.
Sekido Y
Pathology international 2011 Jun;61(6):331-44
Pathology international 2011 Jun;61(6):331-44
Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact.
Leivonen SK, Rokka A, Ostling P, Kohonen P, Corthals GL, Kallioniemi O, Perälä M
Molecular & cellular proteomics : MCP 2011 Jul;10(7):M110.005322
Molecular & cellular proteomics : MCP 2011 Jul;10(7):M110.005322
LATS2 is a tumor suppressor gene of malignant mesothelioma.
Murakami H, Mizuno T, Taniguchi T, Fujii M, Ishiguro F, Fukui T, Akatsuka S, Horio Y, Hida T, Kondo Y, Toyokuni S, Osada H, Sekido Y
Cancer research 2011 Feb 1;71(3):873-83
Cancer research 2011 Feb 1;71(3):873-83
JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis.
Tomlinson V, Gudmundsdottir K, Luong P, Leung KY, Knebel A, Basu S
Cell death & disease 2010;1:e29
Cell death & disease 2010;1:e29
Merlin is a potent inhibitor of glioma growth.
Lau YK, Murray LB, Houshmandi SS, Xu Y, Gutmann DH, Yu Q
Cancer research 2008 Jul 15;68(14):5733-42
Cancer research 2008 Jul 15;68(14):5733-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - YAP1 monoclonal antibody (M01), clone 2F12. Analysis of YAP1 expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody (M01), clone 2F12.Lane 1: YAP1 transfected lysate (Predicted MW: 54.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on HeLa cell. Antibody concentration 30 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml.
