PAB19979

antibody from Abnova Corporation

Targeting: ABHD4 FLJ12816

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB19979

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB19979, RRID:AB_10983736

Product name

ABHD4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ABHD4.

Antigen sequence

ADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARIL
QCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTPL
VMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFG
RSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMI
LLGHSLGGF


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with ABHD4 polyclonal antibody (Cat # PAB19979) at 1:250-1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with ABHD4 polyclonal antibody (Cat # PAB19979) shows moderate cytoplasmic positivity in purkinje cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)