RQ7755

antibody from NSJ Bioreagents

Targeting: TAP1 ABCB2, D6S114E, PSF1, RING4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7755

Provider

NSJ Bioreagents

Product name

Antigen peptide transporter 1 Antibody / Apt1 / Tap1

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Antigen peptide transporter 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat PC-12, 2) rat thymus and 3) mouse RAW264.7 cell lysate with Antigen peptide transporter 1 antibody. Predicted molecular weight ~79 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE mouse RAW264.7 cells with Antigen peptide transporter 1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse HEPA1-6 cells with Antigen peptide transporter 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Antigen peptide transporter 1 antibody.