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LearnH00000529-M02
antibody from Abnova Corporation
Targeting: ATP6V1E1
ATP6E, ATP6E2, ATP6V1E, P31, Vma4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00000529-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000529-M02, RRID:AB_10628559
- Product name
- ATP6V1E1 monoclonal antibody (M02), clone 4E11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant ATP6V1E1.
- Antigen sequence
MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAE
EEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKI
QMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKV
VKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQD
FPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPE
DIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPE
VRGALFGANANRKFLD- Isotype
- IgG
- Antibody clone number
- 4E11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ATP6V1E1 expression in transfected 293T cell line by ATP6V1E1 monoclonal antibody (M02), clone 4E11.Lane 1: ATP6V1E1 transfected lysate(26.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ATP6V1E1 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ATP6V1E1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
