Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29420 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (90%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PLK2 Polyclonal Antibody (Product # PA5-29420). Sample (30 µg of whole cell lysate). Lane A: A549. 7.5% SDS PAGE. PLK2 Polyclonal Antibody (Product # PA5-29420) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PLK2 Polyclonal Antibody detects PLK2 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PLK2 Polyclonal Antibody (Product # PA5-29420) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.