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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX100084 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100084, RRID:AB_1241252
- Product name
- RecQ1 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- RecQ1 antibody [N1N2], N-term detects RecQ1 protein by Western blot analysis.A. 30 ?g A431 whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extractC. 30 ?g A375 whole cell lysate/extract7.5 % SDS-PAGERecQ1 antibody [N1N2], N-term (GTX100084) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RecQ1 antibody [N1N2], N-term detects RecQ1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RecQ1 protein stained by RecQ1 antibody [N1N2], N-term (GTX100084) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of RecQ1 protein from HeLa whole cell extracts using 5 £gg of RecQ1 antibody [N1N2], N-term (GTX100084).Western blot analysis was performed using RecQ1 antibody [N1N2], N-term (GTX100084).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RecQ1 antibody [N1N2], N-term detects RecQ1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RecQ1 antibody [N1N2], N-term (GTX100084) diluted at 1:250.
