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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27099 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RecQ1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, A375.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RecQ1 Polyclonal Antibody detects RecQ1 protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract. C. 30 µg A375 whole cell lysate/extract.7.5 % SDS-PAGE. RecQ1 Polyclonal Antibody (Product # PA5-27099) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RecQ1 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RecQ1 polyclonal antibody (Product # PA5-27099) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RecQ1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RecQ1 Polyclonal Antibody (Product # PA5-27099) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RecQ1 Polyclonal Antibody detects RecQ1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RecQ1 Polyclonal Antibody (Product # PA5-27099) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RecQ1 was performed in HeLa whole cell extracts using 5 µg of RecQ1 Polyclonal Antibody (Product # PA5-27099). Samples were transferred to a membrane and probed with RecQ1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
