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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- NB100-619 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-619, RRID:AB_10002777
- Product name
- Rabbit Polyclonal RECQ1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Human proteins that interact with RNA/DNA hybrids.
RECQ1 plays a distinct role in cellular response to oxidative DNA damage.
Wang IX, Grunseich C, Fox J, Burdick J, Zhu Z, Ravazian N, Hafner M, Cheung VG
Genome research 2018 Sep;28(9):1405-1414
Genome research 2018 Sep;28(9):1405-1414
RECQ1 plays a distinct role in cellular response to oxidative DNA damage.
Sharma S, Phatak P, Stortchevoi A, Jasin M, Larocque JR
DNA repair 2012 Jun 1;11(6):537-49
DNA repair 2012 Jun 1;11(6):537-49
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RECQ1 Antibody [NB100-619] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RecQ1 antibody NB100-619 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 1 second.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RECQ1 Antibody [NB100-619] - Detection of human RecQ1 by Western blot using NB100-619 and human RecQ1 by immunoprecipitation. RecQ1 was immunoprecipitated poorly by NB100-618. Detection: ECL with a 30 second exposure.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RECQ1 Antibody [NB100-619] - RECQ1 immunoprecipitation in human b-cells. Lane 1: 40 ug input, Lane 2: IgG, Lane 3: IP. Antibody at 10 ug/mg lysate. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RECQ1 Antibody [NB100-619] - Detection of human RecQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RecQ1 antibody NB100-619 used for IP at 6 ug per reaction. RecQ1 was also immunoprecipitated by rabbit anti-RecQ1 antibody NB100-618. For blotting immunoprecipitated RecQ1, NB100-619 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RECQ1 Antibody [NB100-619] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RecQ1 at 1:5000 (0.2 ug/mL). Detection: DAB
