PA5-27460

antibody from Invitrogen Antibodies

Targeting: ADD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27460

Provider

Invitrogen Antibodies

Product name

alpha Adducin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, Jurakt, Raji.

Concentration

1.02 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha Adducin Polyclonal Antibody (Product # PA5-27460). Sample (30 µg of whole cell lysate). Lane A: Jurakt . 7.5% SDS PAGE. alpha Adducin Polyclonal Antibody (Product # PA5-27460) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Adducin in methanol-fixed A431 cells using an Alpha Adducin polyclonal antibody (Product # PA5-27460) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Adducin Polyclonal Antibody detects alpha Adducin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alpha Adducin Polyclonal Antibody (Product # PA5-27460) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Adducin Polyclonal Antibody detects alpha Adducin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alpha Adducin Polyclonal Antibody (Product # PA5-27460) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.