PA5-16514

antibody from Invitrogen Antibodies

Targeting: MMP14 MT1-MMP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-16514

Provider

Invitrogen Antibodies

Product name

MMP14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA5-16514 targets MMP-14 in WB applications and shows reactivity with mouse and Human samples. The PA5-16514 immunogen is a synthetic peptide from the hemopexin-like domain of human MMP-14 / MT1-MMP.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Concentration

1 mg/mL

Storage

4° C

References

Submitted references

Effects of excess thromboxane A2 on placental development and nutrient transporters in a Mus musculus model of fetal growth restriction.

Gibbins KJ, Gibson-Corley KN, Brown AS, Wieben M, Law RC, Fung CM
Biology of reproduction 2018 May 1;98(5):695-704

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of MMP-14 using MMP-14 Polyclonal Antibody (Product # PA5-16514) on HFL-1+TPA med Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HT-1080 (Lane 1), MDA-MB-231 (Lane 2) and A549 (Lane 3). The blot was probed with Rabbit Anti-MMP14 Polyclonal Antibody (Product # PA5-16514, 2µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 65 kDa band corresponding to MMP14 was observed across the cell lines tested, an additional band of 160 kDa corresponding to the oligomer form of MMP-14 was also detected. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).