NBP2-22534
antibody from Novus Biologicals
Targeting: CXCL11
b-R1, H174, I-TAC, IP-9, SCYB11, SCYB9B
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- NBP2-22534 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CXCL11/I-TAC Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 200 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CXCL11/I-TAC Antibody (22F1) [NBP2-22534] - Analysis of 0.25 ug of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CXCL11/I-TAC Antibody (22F1) [NBP2-22534] - Human ITAC was performed by coating a blank 96-well microtiter plate with 100ul per well of Human ITAC monoclonal antibody in duplicate at 1, 3, 4, 5, 7, and 9ug/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300ul per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human ITAC recombinant protein at 50ul per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50ul of Human ITAC biotinylated monoclonal antibody in all applicable wells at 0.2ug/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer and incubated with 100ul per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100ul per well of TMB substrate for 30 minutes at room temperature in the dark.